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Research Article

Table 1

Summary of treatments performed in study mice.

Group Treatments (of 100 μL PBS) Total sample size Route of administration Sample size in HIa Sample size in CMIb Sample size in challenges
I Control 25 3 9 10c, 6d
II 100 μL PBS 25 Thigh muscle 3 9 10c, 6d
III 100 μg pVAX1 25 Thigh muscle 3 9 10c, 6d
IV 100 μg pVAX-IL33 25 Thigh muscle 3 9 10c, 6d
V 100 μg pVAX-ROP5 25 Thigh muscle 3 9 10c, 6d
VI 100 μg pVAX-ROP18 25 Thigh muscle 3 9 10c, 6d
VII 50 μg pVAX-ROP5 + 50 μg pVAX-ROP18 25 Thigh muscle 3 9 10c, 6d
VIII 33 μg pVAX-IL33 + 33 μg pVAX-ROP5 + 33 μg pVAX-ROP18 22 Thigh muscle 3 9 10c, 6d
a

To assess humoral immunity (HI), sera were collected from the tail vein prior to immunization from three mice per group.

b

To assess cell-mediated immunity (CMI), through the lymphocyte proliferation assay (n = 3 mice), cytokine measurements (n = 3 mice), and flow cytometric analysis (n = 3 mice), spleens were aseptically removed from nine mice per group two weeks after the last immunization.

c

Two weeks after the final inoculation, the mice in all groups were challenged intraperitoneally with 1 × 103 tachyzoites of T. gondii RH strain.

d

Two weeks after the final inoculation, the mice in all groups were challenged intragastrically with 20 cysts of T. gondii PRU strain.

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