Volume 24, 2017
|Number of page(s)||11|
|Published online||27 November 2017|
Comparison of the in vitro anthelmintic effects of Acacia nilotica and Acacia raddiana
Comparaison des effets anthelminthiques in vitro d'Acacia nilotica et Acacia raddiana
Laboratoire de Biologie et Santé Animales-DPA/INERA,
04 BP 8645
04, Burkina Faso
b Université de Ouagadougou / UFR-SVT, 03 BP 7021 Ouagadougou 03, Burkina Faso
c Instituto de Zootecnia (SAA, APTA), Rua Heitor Penteado 56, Nova Odessa, SP, cep 13460-000, Brazil
d Universidade Federal do Maranhão − UFMA, Campus do Bacanga CEP 65080-805 São Luís- MA, Brazil
e Université Nazi Boni, Bobo-Dioulasso, 01 BP 3770 Ouagadougou 01, Burkina Faso
f Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, NAPTSA, CP 96, CEP 13.400-970, Piracicaba, SP, Brazil
g UMR1213 Herbivores, INRA − Clermont-Ferrand, Vetagro Sup, 63122 Saint-Genès-Champanelle, France
h UMR IHAP 1225 INRA/ENVT, 23 Chemin des Capelles, 31076 Toulouse Cedex, France
* Corresponding author: email@example.com
Accepted: 20 October 2017
Gastrointestinal nematodes are a major threat to small ruminant rearing in the Sahel area, where farmers traditionally use bioactive plants to control these worms, including Acacia nilotica and Acacia raddiana. The main aim of this study was to screen the potential anthelmintic properties of aqueous and acetone extracts of leaves of these two plants based on three in vitro assays: (1) the egg hatch inhibition assay (EHA); (2) the larvae exsheathment inhibition assay (LEIA) using Haemonchus contortus as a model; and (3) an adult mortality test (AMT) applied on Caenorhabditis elegans. For the EHA, only A. raddiana was effective with IC50 = 1.58 mg/mL for aqueous extract, and IC50 = 0.58 mg/mL for acetonic extract. For the LEIA, all extracts inhibited the exsheathment of larvae compared to the controls, and the aqueous extract of A. nilotica was more larvicidal with IC50 = 0.195 mg/mL. In general, all responses to the substances were dose-dependent and were significantly different from the control group (p < 0.05). For the AMT, the extracts of the two Acacia species were effective but A. raddiana showed greater efficacy with 100% mortality at 2.5 mg/mL and LC50 = 0.84 mg/mL (acetonic extract). The addition of polyvinyl polypyrrolidone (PVPP) to the extracts suggested that tannins were responsible for blocking egg eclosion and inducing adult mortality but were not responsible for exsheathment inhibition. These results suggest that the leaves of these Acacia species possess ovicidal and larvicidal activities in vitro against H. contortus, and adulticidal effects against C. elegans.
Les nématodes gastro-intestinaux demeurent une menace majeure de l'élevage des petits ruminants en zone sahélienne, où les éleveurs utilisent traditionnellement des plantes bioactives pour maîtriser ces parasites, notamment Acacia nilotica et Acacia raddiana. L'objectif de ce travail était de vérifier l'efficacité anthelminthique d'extraits aqueux et acétoniques de feuilles de ces deux plantes sur la base de 3 tests in vitro : 1) l'inhibition de l'éclosion des œufs (EHA), 2) l'inhibition du dégainement des larves (LEA) appliquée au modèle Haemonchus contortus et 3) la mortalité des Caenorhabditis elegans adultes (AMT). Pour EHA, seul A. raddiana a été efficace avec IC50 = 1,58 mg/mL pour l'extrait aqueux et IC50 = 0,58 mg/mL pour l'extrait acétonique. Pour LEA, tous les extraits ont inhibé le dégainement des larves par rapport aux témoins et l'extrait aqueux d'A. nilotica a été plus larvicide avec un IC50 = 0,195 mg/mL. De façon générale, toutes les concentrations agissent de façon dose-dépendante et sont significativement différentes du groupe témoin (P < 0,05). Pour AMT, les extraits des deux Acacia ont été efficaces mais A. raddiana a été plus efficace avec 100% de mortalité à 2,5 mg/mL avec une DL50 = 0,84 mg/mL (extrait acétonique). L'addition de polyvinylpolypyrrolidone (PVPP) aux extraits a montré que les tannins seraient responsables de l'inhibition de l'éclosion des œufs et de la mortalité chez les vers adultes, mais ne seraient pas responsables de l'inhibition du dégainement des larves. Ces résultats suggèrent que les feuilles de ces deux Acacia possèdent des activités ovicides et larvicides in vitro contre H. contortus et des effets sur les C. elegans adultes.
Key words: Natural products / tannins / secondary plant metabolites / nematodes / anthelmintics
© G. Zabré et al., published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Small ruminant rearing contributes strongly to human livelihoods in developing countries, especially for rural farmers . In Sahelian countries, such as Burkina Faso, Mali and Niger, livestock play an important role in the economy . In Burkina Faso, the livestock sector alone contributes over USD 51.3 million annually to the national economy, of which 32% is from small ruminants . Despite the importance of this sector, small ruminant rearing faces feeding and health constraints that limit animal production. Among the health constraints, gastrointestinal nematodes (GINs), and Haemonchus contortus in particular (a highly prevalent and pathogenic species of GIN [15,56]), lead to low performances and higher animal mortality [35,58,67]. Like in many developing countries, the majority of livestock owners in rural areas do not use synthetic anthelmintic (AH) drugs because they are not affordable [31,55]. Therefore, they usually rely on practices related to ethnoveterinary medicine to control health problems in livestock . In the Sahel, many natural fodder plants such as different species of Acacia, are used to control disease in ruminants, especially GINs. The use of such bioactive plants has several advantages for local farmers: (a) they are locally available; (b) they cost less compared to synthetic AH drugs; and (c) they are well accepted and consumed by sheep and goats.
An ethnoveterinary survey performed in this area of the Sahel showed that among Acacia species targeted for ruminants, Acacia nilotica var adansonii (Guill. & Perr.) O. Ktze and Acacia raddiana (Savi) are commonly used because of their high nutritional value and their potential AH effects. . All of the breeders investigated used A. raddiana essentially as feed for cattle. However, 57% and 43% of the breeders questioned the use of A. nilotica to treat and to feed the cattle, respectively. For example, decoctions prepared from the pods and dried leaves of A. nilotica are used to treat foot-and-mouth disease (98%), diarrhoea (73%), leg ulcers (51%), loss of appetite (36%) and gingivitis (32%) in small ruminants . In Mauritania, the powdered seed of A. nilotica macerated in fresh water is used to treat diarrhoea. The fruit, leaves and bark extracts of A. nilotica have been examined in in vitro studies [11,12,34,45] and their effects confirmed in in vivo studies [11,12,46]. However, information on A. raddiana is sparse.
Both A. nilotica and A. raddiana species are assumed to contain tannins . Many in vitro and in vivo studies have now provided consistent evidence to support the AH effect of feed containing tannins and other polyphenols against abomasal and intestinal parasitic nematodes [27,29]. These previous investigations have shown that some local plants that contain tannins can impair different key biological processes of the parasitic nematode life cycle: (i) the establishment of the infective third-stage larvae [18,20,47]; (ii) the excretion of eggs by adult worms [41,47,66] and (iii) the development of nematode eggs into larvae , and can therefore be used as nutraceuticals .
The objectives of this study were therefore two-fold: (1) to evaluate, based on in vitro assays, the anthelmintic effects of both aqueous and acetonic extracts of the two Acacia plants on two different development stages of H. contortus and on adult nematode mortality of members of Caenorhabditis elegans; (2) to confirm the possible role of tannins and related polyphenols on in vitro AH effects using the tannin inhibitor polyvinyl polypyrrolidone (PVPP).
Fresh leaves of A. nilotica and A. raddiana were collected in December 2014 at Dori (14° and 15° N; 0° and 3° W), located in north-eastern Burkina Faso. The climate of the region, classified as Sahelian, is marked by a long dry season from November to June and a short rainy season from July to September. The specimens were identified by reference to the herbarium of the French National Centre of Scientific and Technological Research in Ouagadougou, Burkina Faso. Harvested leaves were then cleaned with water and dried at room temperature for a week according to the procedure used by traditional healers in the country.
Dried leaves were first transformed into powders using a blade grinder (basic IKA A 11) and the water content was determined according to the Association of Analytical Communities (AOAC) . Two grams of each powder were dried at 105 °C for 3 h and cooled in a desiccator for 30 min before being weighed. The percentage of water content was calculated according to the following formula: % water content = ((powders before drying (g) − powders after drying (g))/powders before drying (g)) x100.
Two extracts were prepared for each Acacia species: one aqueous and one water/acetonic extract. For aqueous extracts, 150 g of each powdered material were macerated with 750 mL of distilled water for 24 h. Macerated extracts were then filtered over cotton wool and concentrated under reduced pressure in a rotary evaporator at 40-50 °C before being stored at 4 °C. For water/acetonic extracts, 150 g of each powder were macerated with 750 mL of mixed acetone/water (70/30) (v/v) for 72 h. Macerated extracts were filtered over Whatman paper before being concentrated under reduced pressure in a rotary evaporator at 40-50 °C and stored at 4 °C. Dry extracts were subjected to phytochemical screening in order to identify the main phytochemical groups.
Total tannins (TT), total phenols (TP) and condensed tannins (CT) were determined according to Makkar  and Makkar et al. . TP and TT were determined by adding 250 µL of Folin-Ciocalteu reagents (1N) and 1.25 mL sodium carbonate solution (20% Na2CO3) to an aliquot of the supernatant and then taking absorbance readings at 725 nm. To determine TT, a binding tannin agent, 100 mg of insoluble polyvinyl polypyrrolidone (PVPP), was added to the extract and the measurement was repeated. A calibration curve was prepared from aliquots of the solution of tannic acid. The difference between measurements of TP and TP + PVPP extract readings was an estimate of TT. The concentrations of TP and TT were calculated as tannic acid equivalents (eq) and expressed as g/kg DM. CT were expressed as leucocyanidin equivalents (% of DM) and were determined using 3 mL butanol-HCl reagent and 0.1 mL ferric reagent. The absorbance was read at 550 nm.
The faecal matter of a donor goat experimentally infected with H. contortus was collected at the Department of Pathology at the Federal University of Maranhão (UFMA) (Brazil) to obtain the eggs and L3 larvae of H. contortus. C. elegans adult nematodes were obtained by culture according to the method of Chitwood and Feldlaufer . All procedures were approved by the Ethics Committee for Animal Experimentation of the Federal University of Maranhão under number 23115018061.
Three different tests were performed: the egg hatching assay (EHA), the L3 larval exsheathment assay (LEIA) of H. contortus, and the C. elegans adult mortality assay (AMT).
This assay was performed according to the method described by Coles et al. . The fresh faecal matter was recovered, crushed and filtered four times on 1-mm, 105-µm, 55-µm and 25-µm sieves. Eggs collected from the 25-µm sieve were centrifuged at 450 g /20 °C for 10 min and the supernatant was removed and replaced by an NaCl solution (density: 1.2), mixed and centrifuged at 1012 g /20 °C for 5 min. The recovered supernatant was filtered with a 25-μm sieve, washed and centrifuged three times at 450 g /20 °C for 10 min. The quantity was adjusted to reach a concentration of 100 eggs/mL. Five increasing concentrations of solutions were prepared for each extract: 0.3, 0.6, 1.25, 2.5 and 5.0 mg/mL diluted in PBS (0.1 M phosphate, 0.05 M NaCl, pH 7.2) for the two aqueous extracts, and in 2% methanol for the acetonic extracts. To test the direct effect of the extracts on nematodes, 100 µL of each concentration + 100 µL of eggs were placed in each well (96-well plate). Four replicates were performed per concentration. The plates were covered with Parafilm and incubated at 27 °C for 48 h. After 48 h of incubation, hatching was stopped by adding lugol iodine solution, and the number of L1 larvae and eggs per well was counted using a reverse microscope (magnification x 10). Thereafter, the percentage of hatched eggs was determined using the following ratio: number of L1/(number of eggs + number of L1).
The assay on larval exsheathment inhibition was performed as previously described by Bahuaud et al. . Larvae were obtained by larval culture from goat faecal matter incubated at room temperature for 14 days to obtain L3 larvae at the concentration of 2000 larvae/mL. Five increasing concentrations were prepared for each of the two extracts: 2.4, 1.2, 0.6, 0.3 and 0.15 mg/mL, all diluted in phosphate-buffered saline (PBS) (0.1 M phosphate, 0.05 M NaCl, pH 7.2) for aqueous extracts and 1% methanol for acetonic extracts. PBS and 1% methanol were used as a negative control. Briefly, 1 mL of L3 larvae solution was incubated with 1 mL of extract at 20 °C for 3 h, and then washed with PBS and centrifuged three times at 1380 g for 3 min. Then, 200 µL of the larvae were subjected to the artificial exsheathment process by adding 200 µL of a Milton solution (2% w/v sodium hypochlorite and 16.5% sodium chloride) diluted to 1/300 with PBS. Four replicates were performed per concentration. The kinetics of larval exsheathment in the different experimental treatments were then monitored at 0, 20, 40 and 60 min intervals by microscopic observations (40×).
Adult C. elegans were isolated according to Katiki et al. . The test was performed with young adults and adults with intact cuticles. Five concentrations were prepared for the assay: 0.6, 1.25, 2.5, 5 and 10 mg/mL and two negative controls: salt solution M-9 (1.5 g KH2PO4; 3 g Na2HPO4; 2.5 g NaCl; 0.5 mL 1M MgSO4, final volume of 500 mL) for aqueous extracts, and 2% dimethyl sulfoxide (DMSO) for acetonic extracts used as a solvent. The test was performed using 48-well plates and a nematode stock of 50 larvae/100 µL. In each well, nematodes were placed in contact with each concentration to a final volume of 500 µL/well with four replicates. The plates were then incubated at 25 °C for 20 h. After incubation, plates were read using a microscope and all adult worms were counted and determined as dead or alive, according to Skantar et al. . They were considered dead when they did not show any movement and as alive when there were at least some tails, head or pharyngeal movements (during 10 s of observation). The negative control consistently showed 95-100% live adults at 20 h after incubation.
In order to confirm the role of tannins on the AH effect, another series of EHA, LEIA and AMT was performed with both extracts of each Acacia species. However, for EHA, only A. raddiana extracts were used for this test because A. nilotica was not effective. Thus, the best concentrations of each extract (5 mg/mL + 100 mg of PVPP for EHA, 10 mg/mL + 200 mg of PVPP for AMT and 1.2 mg/mL + 250 mg of PVPP for LEIA) were pre-incubated with PVPP for 2 h, centrifuged at 1012 g / 10 min at 20 °C before being used for the assay. After centrifugation, all supernatants were removed to perform the three assays according to the procedure previously described and the results compared to an assay without the addition of PVPP. The ratio of pre-incubated PVPP was 1/10 for EHA and AMT. The rate for LEIA was 1/40.
Data from parasite tests were recorded on Excel 2010 (Microsoft corporation) and transformed to log (x + 1) before being subjected to a variance analysis with SAS software 2010, Version 6.20.4. IC50 and LC50 were calculated by probit analysis. IC50 and LC50 were considered significantly different when the 95% LC fails to overlap . Comparison of averages (expressed as mean ± standard error of mean) was performed using the Kruskal-Wallis test at 0.05.
Table 1 presents the content of total phenol (TP), condensed tannins (CT) and total tannins (TT) for both Acacia species. A. nilotica was rich in CT compared to A. raddiana which was rich in TT and TP.
Quantity of condensed tannin (CT), total tannin (TT) and total polyphenols (TP) in Acacia species.
Tables 2 and 4 show the percentage of inhibition of egg hatching at different concentrations and 50% inhibition concentration for H. contortus. In general, the two controls recorded the lowest percentages of inhibition (4.3 and 9.8%, respectively, for PBS and 2% methanol) and were significantly different for all extracts (p < 0.05). The inhibition of egg hatching increased with increasing concentrations. However, A. nilotica was not effective and recorded a percentage of inhibition of less than 22% and an IC50>5 mg/mL. The percentage of egg inhibition of A. raddiana ranged from 12.67% to 92.76% with an IC50 = 1.58 mg/mL for the aqueous extract, and from 27.34% to 91.45% for acetonic extract with an IC50 = 0.58 mg/mL.
Percentages of egg hatch of H. contortus after 48 h incubation with Acacia nilotica and/or Acacia raddiana extract at different concentrations.
Inhibitory concentrations (mg/mL) in the EHA and LEIA (mg/mL) with Haemonchus contortus (IC50) and lethal concentrations (mg/mL) for the AMT with Caenorhabditis elegans (LC50) with respective 95% confidence intervals for the different plant extracts.
Both extracts revealed inhibition activity on L3 larvae exsheathment of H. contortus. The aqueous extract of A. nilotica showed the lowest concentration of inhibition (IC50) compared to all of the extracts (Table 4).
Tables 3 and 4 present the efficacy of the extracts on adult C. elegans mortality and the lethal concentration. In general, the two controls recorded the lowest mortality rate (4.69% and 0.84%, respectively, for M-9 and 2% DMSO). The differences between concentrations and controls were highly significant (p < 0.001). Moreover, both extracts induced adult C. elegans mortality. When the concentration increased, the percentage of adult mortality increased only for the acetonic extract of A. raddiana, which reached 100% mortality at 2.5 mg/mL with the lowest lethal concentrations: LC50 = 0.84 mg/mL compared to the other extracts.
Percentage of adult Caenorhabditis elegans mortality after 20 h of incubation with Acacia nilotica and Acacia raddiana extract at different concentrations.
The addition of PVPP to the two extracts of each species significantly affected (p < 0.05) egg hatching of H. contortus (Table 5) and adult C. elegans mortality (Table 6). In contrast, for larval exsheathment, the results seem to indicate that incubation of extracts with PVPP did not change the exsheathment process when compared to non-treated extracts (p > 0.05) (Fig. 1).
Inhibition of egg hatching after 48 h incubation with extracts treated or not treated with polyvinyl polypyrrolidone (PVPP) for Acacia raddiana aqueous and acetonic extracts at a concentration of 5 mg/mL.
Adult Caenorhabditis elegans mortality percentage, after incubation for 20 h with Acacia raddiana and Acacia nilotica aqueous and acetonic extracts previously treated or not with polyvinyl polypyrrolidone (PVPP).
Larval exsheathment of H. contortus in the presence of acacia extracts at a concentration of 1.2 mg/mL, and its combination with polyvinyl polyvinylpyrrolidone (PVPP).
The use of tannin-rich plants as an alternative treatment to chemical anthelmintics is one approach that could reduce the development of parasite resistance [32,34,57]. The objective of this study was to evaluate and compare the in vitro ovicidal and larvicidal efficacy of aqueous and acetonic leaf extracts of A. nilotica and A. raddiana against H. contortus, because these two Acacia species are widely used by Sahelian breeders in ethnoveterinary medicine. According to the literature, many species of Acacia have been reported to have anthelmintic activities: the leaves of Acacia cyanophylla , Acacia karoo , Acacia nilotica [34,45], Acacia pennatula [2,3] and Acacia polyancatha , and the bark of Acacia mangium  and Acacia mearrsii .
In our studies, three in vitro assays were performed with two extracts (aqueous and acetonic extracts) at different concentrations. These types of extracts have been commonly used in many in vitro tests. Moreover, solvents and protocols used for extraction caused variations in concentrations and the classes of metabolites present in extracts .
The quantification of tannins revealed that A. nilotica was 18 times richer in CT than A. raddiana. However, A. raddiana was seven times richer in TT and five times richer in TP than A. nilotica. According to Hoste et al. , tannin-rich plants could interact with the proteins of the cuticle, oral cavity, oesophagus, cloaca and vulva of nematodes, modifying their chemical and physical properties. The anthelmintic effects of tannins may be attributed to their capacity to bind free protein available in the tubes for larval nutrition, and this reduced nutrient availability could therefore have resulted in larval starvation or decrease in gastrointestinal metabolism directly through the inhibition of oxidative phosphorylation, causing larval death .
Our studies conducted in vitro with A. nilotica and A. raddiana extracts showed the inhibition action on egg hatching and larval exsheathment of H. contortus and mortality of C. elegans adults. Extracts acted in a dose-dependent manner and their efficacy differed depending on the organ. This anthelmintic efficacy may be attributed to an individual or a combined effect of the bioactive compounds.
In the EHA, A. nilotica extracts were not effective compared to A. raddiana. A. nilotica inhibited 22% of egg hatching for the most effective concentration (5 mg/mL) with IC50>5 mg/mL. However, the two extracts of A. raddiana presented a high ovicidal activity (more than 90% at 5 mg/mL) with different concentrations of inhibition: IC50 = 0.68 mg/mL for acetonic extract and IC50 = 1.36 mg/mL for aqueous extract. Acetonic extracts were more ovicidal than aqueous extracts, but no significant difference was recorded between the extracts in our study. Thus, the results obtained for A. nilotica contrasted with those obtained by Eguale et al.  who reported significant egg hatching inhibition for aqueous extracts for the same plant. Likewise, Badar et al.  found that crude aqueous methanol extracts of A. nilotica bark (LC50 = 0.201 mg/mL) had higher inhibitory effects compared with leaves (LC50 = 0.769 mg/mL). Other results have confirmed the anthelmintic effect of different Acacia species on EHA. Thus, Oliveira  reported IC50 = 1.35 mg/mL and 4.66 mg/mL with A. mangium extract on two H. contortus (White River: a strain resistant to ivermectin, benzimidazole and closantel; and the Juan strain: susceptible to all synthetic anthelmintics, respectively). Secondary plant metabolites (1) might bind the lipoproteins responsible for eggshell membrane permeability , and (2) tannins might inactivate enzymes responsible for the hatching process .
In the LEIA, both Acacia extracts were effective against larval exsheathment. A. nilotica revealed the lowest concentration of inhibition (IC50 = 0.195 mg/mL) for the aqueous extract compared to the aqueous extract of A. raddiana (IC50 = 0.331 mg/mL). However, the acetonic extract of A. nilotica was numerically higher compared to A. raddiana. Moreover, all extracts at 600 and 1200 μg/mL blocked the exsheathment process. Previous in vitro results indicated that extracts of various tannin-rich woody plants inhibited the exsheathment of nematode L3 . Alonso-Diaz et al. [2,3] tested Acacia pennatula and A. gaumeri extracts and found an inhibition of 51% at 1200 μg/mL, 93.5% at 600 μg/mL, and a total exsheathment process after 70 min at 1.2 mg/mL, respectively. The exsheathment process in trichostrongyle nematodes is a crucial step that represents the transition from the free-living to the parasitic stages.
In the AMT, the free-living soil nematode C. elegans was used to test the efficacy of the extracts. C. elegans is a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including H. contortus . The two Acacia extracts tested were effective against adult C. elegans and their lethal concentrations were less than LC50 < 1.5 mg/mL, except for the acetonic extract of A. nilotica (LC50 = 5.4 mg/mL). For the same concentration (2.5 mg/mL), the acetonic extract of A. raddiana revealed 100% adult mortality compared to 19% for A. nilotica. However, their two aqueous extracts revealed the same percentage of mortality (80.32 and 81.78%, respectively). In general, A. raddiana was more larvicidal than A. nilotica. A. raddiana contained high total tannins (TT). Plant extracts containing hydrolysable tannins (HT) such as gallic and/or ellagic acid or containing prodelphinidin CT (with gallic acid units) had higher levels of anthelmintic activity in vitro than the proanthocyanidin CT, which lack gallic acid units . According to Katiki et al.  plants containing both CT and HT of the gallotannin and ellagitannin types were more lethal to C. elegans than plants containing CT lacking gallic acid units.
The results with PVPP for all assays showed that both extracts of Acacia species may have variability in the roles of tannins for EHA, LEIA and AMT. For our results, the addition of PVPP in Acacia extracts was associated with a significant decrease (restoration of control values) of EHA and AMT, but, did not seem to influence LEIA.
In the AMT, tannins and the associated metabolites seemed responsible for the mortality of adult C. elegans for A. raddiana extracts compared to A. nilotica extracts. Thus, tannins would be responsible for the efficacy of the extracts for the inhibition of egg hatching, but were not the only metabolites involved in adult mortality for A. raddiana extracts. These results suggest that a possible synergistic relationship of tannins and other compounds may be involved in killing the worms. For tannins, including condensed tannins, their anthelmintic activities have been reported by Athanasiadou et al. , Paolini et al. , Min et al. , Hoste et al.  and Gertrude et al. . Tannins would appear to be able to bind to proteins and glycoproteins of the adult cuticle (a structure rich in proline and hydroxyproline), to the enzymes secreted by worms and involved in various essential functions , or to interact with their digestive epithelium to inhibit nutrition and cause the death of the parasite. Similar deductions were made with aqueous and ethanolic extracts of Moringa oleifera , aqueous extracts of Anogeissus leiocarpus and Daniellia oliveri , Leucaena leucocephala and Gliricidia sepium .In our study, the inefficacy of PVPP to restore control values of larval exsheathment suggested that tannins were not the metabolites responsible for anthelmintic activity on H. contortus. According to Chan-Pérez et al.  and Vargas-Magana , tannins were not the sole secondary plant metabolites responsible for the AH effects. Our results contrasted with many previous studies that suspected tannins of being responsive to inhibit exsheathment [2,43,44,52]. Thus, Brunet et al.  and Hoste et al.  observed a structural lesion on H. contortus L3 larvae (in vitro and in vivo) in contact with sainfoin extracts, a tannin-rich plant. Oliveira et al. , showed the inhibition of larval exsheathment in H. contortus subject to A. mangium extract. Son-de Fernex et al.  observed an inhibition effect on exsheathment of H. contortus larvae subjected to five plants containing CTs. Consequently, we think that other metabolites besides tannins would be able to affect the exsheathment kinetics of L3 larvae by binding to proteins and glycoproteins of the sheath to prevent the formation of the indented ring in the anterior part.
The work of Barrau et al.  and Ayers et al.  showed that flavonoids could play an essential role in the anthelmintic activity of some plants. Thus, the sheath of infective larvae of parasitic nematodes would be the target of flavonoids [10,19] as well as polyphenols . Bizimenyera et al.  showed that polyphenols could have anthelmintic activities on nematodes. These chemical groups may be able to inhibit the secretion of proteases and acetyl-cholinesterase fluid by the glandular cells of the larvae to thereby prevent digestion and separation of the cap from the rest of the sheath.
However, other bioactive compounds and secondary plant metabolites could interact with multiple molecular targets on the various developmental stages of the parasite. Nandi et al.  showed that saponins have the ability to generate ions and cause lipid peroxidation of egg membranes, as well as to cause damage at the larval cuticle to inhibit parasite development. Other authors suggest that the conjugated unsaturated system of the saponins is involved in producing their damaging effect, probably resulting in free radicals, which induce membrane damage through peroxidation in nematodes .
A. nilotica and A. raddiana extracts both have in vitro anthelmintic activities against H. contortus and C. elegans. The two species of Acacia had completely different tannin contents. A. nilotica, rich in CT, was (1) not effective in inhibiting egg hatching; (2) partially effective for adult mortality; and (3) effective in inhibiting larval exsheathment. However, A. raddiana, rich in TT and TP, was highly effective in the three assays. Thus, in our study, TT-rich plants showed higher and more diverse anthelminthic activities against H. contortus compared to CT-rich plants. HT and CT are both found in TT. It is therefore possible that HT are responsible for the efficacy of A. raddiana extracts. Moreover, HT are known to show some toxicity for the ruminants, but the leaves of this plant were consumed by small ruminants in the Sahelian region of Burkina Faso. It is possible that the types and the quantity of HT contained in the leaves were not sufficient to cause ruminal toxicity but could affect the parasites. Future studies are required to ascertain which types or HT (gallotannins or ellagitannins) are contained in A. raddiana extract and underlie the anthelminthic activities. In general, the two species of Acacia used in our study were effective against H. contortus. Therefore, the association of these Acacia in the treatment of gastrointestinal parasites in a traditional environment might be a valuable approach. However, it would be necessary to conduct in vivo parasitological studies to consider the metabolism of extracts in the digestive tract of ruminants.
The authors declare that they have no competing interests.
The authors thank the Tripartite Research Project (Africa − Brazil − France), “Fight against desertification in Africa”, funded by INRA/IRD-AIRD and CNPq 457559/2012-8, for supporting the study, as well as the translator of the manuscript.
- Akkari H, Ben Salem H, Gharbi M, Abidi S, Darghouth MA. 2008a. Feeding Acacia cyanophylla Lindl. foliage to Barbarine lambs with or without PEG: Effect on the excretion of gastro-intestinal nematode eggs. Animal Feed Science and Technology, 147, 182-192. [CrossRef] (In the text)
- Alonso-Díaz MA, Torres-Acosta JFJ, Sandoval-Castro CA, Aguilar-Caballero AJ, Hoste H. 2008a. In vitro larval migration and kinetics of exsheathment of Haemonchus contortus exposed to four tropical tanniniferous plants extracts. Veterinary Parasitology, 153, 313-319. [CrossRef] [EDP Sciences] (In the text)
- Alonso-Díaz MA, Torres-Acosta JFJ, Sandoval-Castro CA, Capetillo- Leal C, Brunet S, Hoste H. 2008b. Effects of four tropical tanniniferous plants on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubriformis infective stage. Veterinary Parasitology, 153, 187-192. [CrossRef] [EDP Sciences] (In the text)
- Alonso-Díaz MA, Torres-Acosta JFJ, Sandoval-Castro CA, Hoste H, Aguilar-Caballero AJ, Capetillo-Leal CM. 2008c. Is goats' preference of forage trees affected by their tannin or fiber content when offered in cafeteria experiments. Animal Feed Science and Technology, 141, 36-48. [CrossRef] (In the text)
- Association pour la promotion de l'élevage en Savane et au Sahel (APSS). 2015. Le paradoxe de l'élevage au Sahel: forts enjeux, faibles soutiens. Inter-Réseaux Développement rural / Bulletin de synthèse souveraineté alimentaire, n° 16. (In the text)
- Association of Official Analytical Chemists (AOAC). 1990. Official Methods of Analysis, vol. II, 15th ed. Arlington, VA, USA. (In the text)
- Athanasiadou S, Kyriazakis I, Jackson F, Coop RL. 2001. The effects of condensed tannins supplementation of foods with different protein content on parasitism, food intake and performance of sheep infected with Trichostrongylus colubriformis. British Journal of Nutrition, 86, 697-706. [CrossRef] (In the text)
- Athanasiadou S, Kyriazakis I, Jackson F, Coop RL. 2001. Direct anthelmintic effects of condensed tannins towards different gastrointestinal species: in vitro and in vivo studies. Veterinary Parasitology, 99, 205-219. [CrossRef] [PubMed] (In the text)
- Ayers S, Zink DL, Mohn K, Powell JS, Brown CM, Murphy T, Brand R, Pretorius S, Stevenson D, Thompson D, Singh SB. 2008. Flavones from Struthiola argentea with anthelmintic activity in vitro. Phytochemistry, 69, 541-545. [CrossRef] [PubMed] (In the text)
- Azando EVB, Hounzangbé-Adoté MS, Olounladé PA, Brunet S, Fabre N, Valentin A, Hoste H. 2011b. Involvement of tannins and flavonoids in the in vitro effects of Newbouldia laevis and Zanthoxylum zanthoxyloïdes extracts on the exsheathment of third-stage infective larvae of gastrointestinal nematodes. Veterinary Parasitology, 180, 292-297. [CrossRef] [PubMed] (In the text)
- Bachaya HA, Iqbal Z, Khan MN, Sindhu ZUD, Jabbar A. 2009. Anthelmintic activity of Ziziphus nummularia (bark) and Acacia nilotica (fruit) against Trichostrongylid nematodes of sheep. Journal of Ethnopharmacology, 123, 325-329. [CrossRef] [PubMed] (In the text)
- Badar N, Iqbal Z, Khan MN, Akhtar M.S. 2011. In vitro and in vivo anthelmintic activity of Acacia nilotica (L.) willd. ex delile bark and leaves. Pakistan Veterinary Journal, 31, 185-191. (In the text)
- Bahuaud D, Martinez-Ortiz MC, Chauveau S, Prevot F, Torres-Acosta F, Fouraste I, Hoste H. 2006. Effects of four tanniferous plant extracts on the in vitro exsheathment of third-stage larvae of parasitic nematodes. Parasitology, 132, 545-554. [CrossRef] [PubMed] (In the text)
- Barrau E, Fabre N, Fouraste I, Hoste H. 2005. Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. Parasitology, 131, 531-538. [CrossRef] [PubMed] (In the text)
- Belem AMG, Kaboré A, Bessin R. 2005. Gastrointestinal helminths of sheep in the central, eastern and northern parts of Burkina Faso. Bulletin of Animal Health and Production in Africa, 53, 13-23. (In the text)
- Bizimenyera ES, Swan GE, Chikoto H, Eloff JN. 2005. Rationale for using Peltophorum africanum (Fabaceae) extracts in veterinary medicine. Journal of South African Veterinary Association, 76, 54-58. [CrossRef] (In the text)
- Brunet S, Hoste H. 2006. Monomers of condensed tannins affect the larval exsheathment of parasitic nematodes of ruminants. Journal of Agricultural and Food Chemistry, 54, 7481-7487. [CrossRef] [PubMed] (In the text)
- Brunet S, Aufrere J, El-babili F, Fourasté I, Hoste H. 2007. The kinetics of exsheathment of infective nematode larvae is disturbed in the presence of a tannin-rich plant extract (sainfoin) both in vitro and in vivo. Parasitology, 134, 1253-1262. [CrossRef] [PubMed] (In the text)
- Brunet S. 2008. Analyse des mécanismes d'action antiparasitaire de plantes riches en substances polyphénoliques sur les nématodes du tube digestifs des ruminants. Thèse de Doctorat, Université Paul Sabatier de Toulouse. 246 pp. (In the text)
- Brunet S, Martínez-Ortiz de Montellano C, Torres-Acosta JFJ, Sandoval-Castro CA, Aguilar-Caballero AJ, Capetillo-Leal C, Hoste H. 2008. Effect of the consumption of Lysiloma latisiliquum on the larval estab-lishment of gastrointestinal nematodes in goats. Veterinary Parasitology, 157, 81-88. (In the text)
- Chan-Pérez JI, Torres-Acosta JFJ, Sandoval-Castro CA, Hoste H, Castañeda-Ramíreza GS, Vilarem G, Mathieu C. 2016. In vitro susceptibility of ten Haemonchus contortus isolates from different geographical origins towards acetone:water extracts of two tannin rich plants. Veterinary Parasitology, 217, 53-60. [CrossRef] [PubMed] (In the text)
- Chitwood DJ, Feldlaufer MFE. 1990. Ecdysteroids in axenically propagated Caenorhabditis elegans and culture medium. Journal of Nematology, 22, 598-607. [PubMed] (In the text)
- Coles GC, Bauer C, Borgsteede FHM, Geerts S, Klei TR, Taylor MA, Waller PJ. 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.). Methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, 44, 35-44. [CrossRef] [PubMed] (In the text)
- Eguale T, Tadesse D, Giday M. 2011. In vitro anthelmintic activity of crude extracts of five medicinal plants against egg-hatching and larval development of Haemonchus contortus. Journal of Ethnopharmacology, 137, 108-113. [CrossRef] [PubMed] (In the text)
- Gertrude MT, Josué WP, Marie-Claire K, Jeannette Y, Alidou MN, Mpoame M. 2014. Anthelminthic activity of Moringa oleifera leaf extracts evaluated in vitro on four developmental stages of Haemonchus contortus from Goats. American Journal of Plant Sciences, 5, 1702-1710. [CrossRef] (In the text)
- Hilou A, Rappez F, Duez P. 2014. Ethnoveterinary management of cattle helminthiasis among the Fulani and the Mossi (Central Burkina Faso): plants used and modes of use. International Journal of Biological and Chemical Sciences, 8, 2207-2221. [CrossRef] (In the text)
- Hoste H, Jackson F, Athanasiadou S, Thamsborg SM, Hoskin SO. 2006. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends in Parasitology, 22, 253-261. [CrossRef] [EDP Sciences] [PubMed] (In the text)
- Hoste H, Torres-Acosta JF, Alonso-Diaz MA, Brunet S, Sandoval-Castro C, Hounzangbé-Adoté S. 2008. Identification and validation of bioactive plants for the control of gastrointestinal nematodes in small ruminants. Tropical Biomedicine, 25, 56-72. [PubMed] (In the text)
- Hoste H, Martínez-Ortiz-De-Montellano C, Manolaraki F, Brunet S, Ojeda-Robertos N, Fourquaux I, Torres-Acosta JFJ, Sandoval-Castro CA. 2012. Direct and indirect effects of bioactive tannin-rich tropical and temperate legumes against nematode infections. Veterinary Parasitology, 186, 18-27. [CrossRef] [PubMed] (In the text)
- Hoste H, Torres-Acosta JFJ, Sandoval-Castro CA, Mueller-Harvey I, Sotiraki S, Louvandini H, Thamsborg SM, Terrill TH. 2015. Tannin containing legumes as a model for nutraceuticals against digestive parasites in livestock. Veterinary Parasitology, 212, 5-17. [CrossRef] [PubMed] (In the text)
- Kaboré A, Tamboura HH, Belem AMG, Traoré A. 2007. Traitements ethno-vétérinaires des parasitoses digestives des petits ruminants dans le plateau central du Burkina Faso. International Journal of Biological and Chemical Sciences, 1, 297-304. (In the text)
- Kaboré A, Belem AMG, Tamboura HH, Traoré A, Sawadogo L. 2009. In vitro anthelmintic effect of two medicinal plants (Anogeissus leiocarpus and Daniellia oliveri) on Haemonchus contortus, an abomasal nematode of sheep in Burkina Faso. African Journal of Biotechnology, 8, 4690-4695. (In the text)
- Kaboré A, Traoré A, Nignan M, Gnanda BI, Bamogo V, Tamboura HH, Bélem AMG. 2012. In vitro anthelminthic activity of Leuceana leucocephala (Lam.) De Wit. (Mimisaceae) and Gliricidia sepuim (Jacq.) Kunth ex Steud (Fabaceae) leave extracts on Hæmonchus contortus ova and larvae. Journal of Chemical and Pharmaceutical Research, 4, 303-309. (In the text)
- Kahiya C, Mukaratirwa S, Thamsborg SM. 2003. Effects of Acacia nilotica and Acacia karoo diets on Haemonchus contortus infection in goats. Veterinary Parasitology, 115, 265-274. [CrossRef] [PubMed] (In the text)
- Kamaraj C, Abdul Rahuman A. 2011. Efficacy of anthelmintic properties of medicinal plant extracts against Haemonchus contortus. Research in Veterinary Science, 91, 400-404. [CrossRef] (In the text)
- Katiki LM, Ferreiara JFS, Zajac AM, Masler C, Lindsay DS, Chagas ACS, Amarante AFT. 2011. Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. Veterinary Parasitology, 182, 264-268. [CrossRef] [PubMed] (In the text)
- Katiki LM, Ferreiara JFS, Gonzalez JM, Zajac AM, Lindsay DS, Chagas ACS, Amarante AFT. 2013. Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity. Veterinary Parasitology, 192, 218-227. [CrossRef] [PubMed] (In the text)
- Makkar HPS, Blummel M, Borowy NK, Becker K. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science of Food and Agriculture, 61, 161-165. [CrossRef] (In the text)
- Makkar HP. 2003. Quantification of tannins in tree and shrub foliage: a laboratory manual. Animal Production and health Section. Joint FAO/IAEA Division of nuclear Technique in Food and Agriculture. International Atomic Energy Agency, Vienna, Austria, 49-53. (In the text)
- Marie-Magdeleine C, Hoste H, Mahieu M, Varo H, Archimede H. 2009. In vitro effects of Cucurbita moschata seed extracts on Haemonchus contortus. Veterinary Parasitology, 161, 99-105. [CrossRef] [PubMed] (In the text)
- Martínez-Ortíz-De-Montellano C, Vargas-Magana JJ, Canul-Ku L, Miranda-Soberanis R, Capetillo-Leal C, Sandoval-Castro CA, Hoste H, Torres-Acosta JFJ. 2010. Effect of a tropical tannin-rich plant, Lysiloma latisiliquum on adult populations of Haemonchus contortus in sheep. Veterinary Parasitology, 172, 283-290. (In the text)
- Max RA, Kimambo AE, Kassuku AA, Mtenga LA, Buttery PJ. 2007. Effects of tanniniferous browse meal on nematode faecal egg counts and internal parasite burdens in sheep and goats. South African Journal of Animal Science, 37, 97-106. (In the text)
- Molan AL, Waghorn GC, Mcnabb WC. 2002. Effect of condensed tannins on egg hatching and larval development of Trichostrongylus colubriformis in vitro. Veterinary Record, 150, 65-69. [CrossRef] (In the text)
- Molan AL, Faraj AM. 2010. The effects of condensed tannins extracted from different plant species on egg hatching and larval development of Teladorsagia circumcincta (Nematoda: Trichostrongylidae). Folia Parasitologica, 57, 62-68. [CrossRef] [PubMed] (In the text)
- Moreno FC, Gordon IJ, Wright AD, Benvenutti MA, Saumell CA. 2010. In vitro anthelmintic effect of plant extracts against infective larvae of ruminants gastrointestinal nematode parasites. Archivos de Medicina Veterinaria, 42, 155-163. [CrossRef] (In the text)
- Moreno FC, Gordon IJ, Knox MR, Summer PM, Skerrat LF, Benvenutti MA, Saumell CA. 2012. Anthelmintic efficacy of five tropical native Australian plants against Haemonchus contortus and Trichostrongylus colubriformis in experimentally infected goats (Capra hircus). Veterinary Parasitology, 187, 237-243. [CrossRef] [PubMed] (In the text)
- Moreno-Gonzalo J, Osoro K, García U, Frutos P, Celaya R, Ferreira LMM, Ortega-Mora LM, Ferre I. 2013. Effect of the consumption of heather on incoming larvae and established population of Teladorsagia circumcincta in experimentally infected Cashmere goats. Veterinary Parasitology, 196, 124-129. [CrossRef] (In the text)
- Min BR, Barry TN, Attwood GT, McNabb WC. 2003. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Animal Feed Science and Technology, 106, 3-19. [CrossRef] [EDP Sciences] (In the text)
- Ministère des Ressources Animales (MRA), 2012. Statistique du secteur de l'élevage. Annuaire 2011. Ouagadougou. 151 p. (In the text)
- Nandi B, Roy S, Bhattacharya S, Babu SPB. 2004. Free radicals mediated membrane damage by the saponins Acacia side A and Acacia side B. Phytotherapy Research, 18, 191-194. [CrossRef] (In the text)
- Niezen JH, Waghorn GC, Graham T, Carter JL, Leathwick DM. 2002. The effect of diet fed to lambs on subsequent developmentof Trichostrongylus colubriformis larvae in vitro and on pasture. Veterinary Parasitology, 105, 269-283. [CrossRef] [PubMed] (In the text)
- Olivera LMB, Bevilaqua CML, Macedo ITF, Morais SM, Monteiro MVB, Campello CC, Ribeiro WLC, Batista EKF. 2011a. Effects of six tropical plant extracts on larval exsheathment of Haemonchus contortus. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, 20, 155-160. [CrossRef] (In the text)
- Olivera LMB, Bevilaqua CML, Macedo ITF, Morais SM, Machado LKA, Campello CC, Mesquita MA. 2011b. Effects of Myracrodrum urundeuva extracts on egg hatching and larval exsheathment of Haemonchus contortus. Parasitology Research, 109, 893-898. [CrossRef] (In the text)
- Oliveira GC. 2014. Uso de Clitoria fairchildiana Ra Howard Fabaceae e Acacia mangium Willd Mimosaceae sobre trichostrongilídeos de caprinos. 84f. Dissertação (Mestrado em Ciências da Saúde) − Programa de Pós-Graduação em Ciências da Saúde − Universidade Federal do Maranhão, São Luís. (In the text)
- Olounladé AP. 2005. Effets anthelminthiques des feuilles de Newbouldia laevis testées in vivo sur les nématodes gastro-intestinaux (Haemonchus contortus et Trichostrongylus colubriformis) chez les moutons Djallonké. DEA, Université de Lomé, Togo, p. 65. (In the text)
- Ouattara L, Dorchies PH. 2001. Helminthes gastro-intestinaux des moutons et chèvres en zones sub-humide et sahélienne du Burkina Faso. Revue de Médecine Vétérinaire, 152, 165-170. (In the text)
- Paolini V, Bergeaud JP, Grisez C, Prevot F, Dorchies P, Hoste H. 2003. Effects of condensed tannins on goats experimentally infected with Haemonchus contortus. Veterinary Parasitology, 113, 253-261. [CrossRef] [PubMed] (In the text)
- Periasamy K, Pichler R, Poli M, Cristel S, Cetrá B, Medus D, Basar M, Thiruvenkadan AK, Ramasamy S, Ellahi MB, Mohammed F, Teneva A, Shamsuddin M, Podesta MG, Diallo A. 2014. Candidate gene approach for parasite resistance in sheep variation in immune pathway genes and association with fecal egg count. PLoS One 9, e88337 (In the text)
- Perry RN. 2002. Hatching. In: Donald L. Lee (Ed.), The Biology of Nematodes. Taylor and Francis, London and New York, p. 147-170. (In the text)
- Roditakis E, Roditakis NE, Tsagkarakou A. 2005. Insecticide resistance in Bemisia tabaci (Homoptera: Aleyrodidae) populations from Crete. Pest Management Science, 61, 577–582. [CrossRef] [PubMed] (In the text)
- Rubanza CDK, Shem MN, Otsyina R, Bakengesa SS, Ichinohe T, Fujihara T. 2005. Polyphenolics and tannins effect on in vitro digestibility of selected Acacia species leaves. Animal Feed Science and Technology, 119, 129-142 [CrossRef] (In the text)
- Sinha Babu SP, Sarkar D, Ghosh NK, Saha A, Sukul NC, Bhattacharya SH. 1997. Enhancement of membrane damage by saponins isolated from Acacia auriculiformis. Japanese Journal of Pharmacology, 75, 451-454. [CrossRef] (In the text)
- Skantar AM, Agama K, Meyer SLF, Carta LK, Vinyard BT. 2005. Effects of geldanamycin on hatching and juvenile motility in Caenorhabditis elegans and Heterodera glycines. Journal of Chemical Ecology, 31, 2481-2491. [CrossRef] (In the text)
- Son-De Fernex EV, Alonso-Diaz MA, Valles-De La Mora B, Capetillo-Leal CM. 2012. In vitro anthelmintic of five tropical legumes on the exsheathment and motility of Haemonchus contortus infective larvae. Experimental Parasitology, 131, 413-418. [CrossRef] (In the text)
- Sujon MA, Mostoja M, Jahan MS, Das AR, Rob S. 2008. Studies on medicinal plants against gastrointestinal nematodes of goats. Bangladesh Journal of Veterinary Medicine, 6, 179-183. (In the text)
- Terril TH, Dykes GS, Shaik SA, Miller JE, Kouakou B, Kannan G, Burke JM, Mosjidis JA. 2009. Efficacy of sericea lespedeza hay as a natural dewormer in goats: Dose titration study. Veterinary Parasitology, 163, 52-56. [CrossRef] [PubMed] (In the text)
- Torres-Acosta JFJ, Hoste H. 2008. Alternative or improved methods to limit gastro-intestinal parasitism in grazing sheep and goats. Small Ruminant Research, 77, 159-173. [CrossRef] (In the text)
- Vargas-Magana JJ, Torres-Acosta JFJ, Aguilar-Caballero AJ, Sandoval-Castro CA, Hoste H, Chan-Pérez JI. 2014. Anthelmintic activity of acetone-water extracts against Haemonchus contortus eggs: Interactions between tannins and other plant secondary compounds. Veterinary Parasitology, 206, 322-327. [CrossRef] [PubMed] (In the text)
- Yoshibara E, Minho AP, Cardim ST, Tabacow VBD, Yamamura M.H. 2014. In vitro ovicidal and larvicidal activity of condensed tannins on gastrointestinal nematode infestations in sheep (Ovis aries). Semina: Ciências Agrárias, 35, 3173-3180. [CrossRef] (In the text)
- Zabré G, Kaboré A, Bayala B, Tamboura HH, Belem AMG, Niderkorn V, Costa Junior ML, Louvandini H, Hoste H. 2017. Botanical and ethnoveterinary surveys of two acacias (Acacia raddiana and Acacia nilotica) exploited in small ruminant rearing in Sahelian area of Burkina Faso. Animal and Veterinary Sciences, 5, 63-68. [CrossRef] (In the text)
Cite this article as: Zabré G, Kaboré A, Bayala B, Katiki LM, Costa-Júnior LM , Tamboura HH, Belem AMG, Abdalla AL, Niderkorn V, Hoste H, Louvandini H. 2017. Comparison of the in vitro anthelmintic effects of Acacia nilotica and Acacia raddiana. Parasite 24, 44
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