Invasion of the ΔHsp90 parasite. The invasion ratio of ΔHsp90, RHΔku80 and complemented parasites was evaluated, the points of time were 1, 2 and 4 h, and anti-SAG3 monoclonal antibody was used as the red-green attachment assay. Fields were randomly selected and data were mean ± SD of three independent experiments. “**” and “*” indicate statistically significant difference at p < 0.01 and p < 0.05, respectively.