Issue |
Parasite
Volume 24, 2017
|
|
---|---|---|
Article Number | 22 | |
Number of page(s) | 11 | |
DOI | https://doi.org/10.1051/parasite/2017023 | |
Published online | 19 June 2017 |
Research Article
The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth
La protéine de choc thermique 90 de Toxoplasma gondii est essentielle pour l’invasion des cellules hôtes et la croissance des tachyzoïtes
1
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou
310058, PR China
2
Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen
518045, PR China
3
Department of Biomedical Sciences and One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre, St. Kitts, West Indies
* Corresponding author: afdu@zju.edu.cn
Received:
24
February
2017
Accepted:
25
May
2017
Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warm-blooded vertebrates. Heat shock proteins (HSP) regulate key signal transduction events in many organisms, and heat shock protein 90 (Hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. Here, we discovered increased transcription of the Hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro, suggesting that Hsp90 may be connected with bradyzoite development in T. gondii. A knockout of the TgHsp90 strain (ΔHsp90) and a complementation strain were constructed. The TgHsp90 knockout cells were found to be defective in host-cell invasion, were not able to proliferate in vitro in Vero cells, and did not show long-time survival in mice in vivo. These inabilities of the knockout parasites were restored upon complementation of TgHsp90. These data unequivocally show that TgHsp90 contributes to bradyzoite development, and to invasion and replication of T. gondii in host cells.
Résumé
Toxoplasma gondii est un Apicomplexa parasite intracellulaire obligatoire qui infecte presque tous les vertébrés à sang chaud. Les protéines de choc thermique régulent les événements principaux de transduction du signal chez de nombreux organismes et la protéine de choc thermique 90 (Hsp90) joue un rôle important dans la croissance, le développement et la virulence chez plusieurs protozoaires parasites. Dans ce travail nous avons découvert une augmentation de la transcription du gène de Hsp90 dans les conditions de différenciation des bradyzoïtes et de chocs alcalins et thermiques in vitro, ce qui suggère que Hsp90 est lié au développement des bradyzoïtes chez T. gondii. Un knock-out de la souche TgHsp90 (ΔHsp90) et une souche de complémentation ont été construits. Les cellules knock-out TgHsp90 se sont révélées défectueuses dans l’invasion des cellules hôtes, n’ont pas pu proliférer in vitro dans des cellules Vero et n’ont pas montré de survie de longue durée chez la souris in vivo. Ces défauts des parasites knock-out ont été restaurés lors de la complémentation de TgHsp90. Ces données montrent sans équivoque que TgHsp90 contribue au développement des bradyzoïtes, et à l’invasion et à la réplication de T. gondii dans les cellules hôtes.
Key words: Toxoplasma gondii / Heat shock protein 90 / Invasion / Differentiation / Replication
© H. Sun et al., published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.