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The relative expression level of BAG1 and MAG1. ΔHsp90, RHΔku80 and complemented parasites (105) were cultured in Vero cells for 24, 48, 72 and 96 h. The RNA was extracted by the Trizol dissociation method and detected by relative SYBR-green real-time PCR using actin transcripts as an internal control. *p < 0.05, **p < 0.01. Data represent mean ± SD, n = 3 experiments.
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