Open Access
Short Note
Issue
Parasite
Volume 23, 2016
Article Number 48
Number of page(s) 5
DOI https://doi.org/10.1051/parasite/2016061
Published online 16 November 2016

© V. Dvorak et al., published by EDP Sciences, 2016

Licence Creative Commons
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction

Phlebotomine sand flies (Diptera: Psychodidae) are vectors of several infectious pathogens including parasitic protozoans of the genus Leishmania and phleboviruses and are therefore of great importance in human and veterinary medicine [5, 11]. Although in Europe they occur typically in the Mediterranean countries, some species extend their range of distribution into regions north of their core areas [12]. As the presence of a vector species is one of the risk factors for Leishmania transmission [19], it is very important to study the limits of sand fly occurrence because their presence in areas at the edge of their distribution range may be overlooked. This study was conducted as part of the VectorNet project, which focuses on mapping sand fly presence in Europe, including the northern limits of their distribution. To pursue this objective, we surveyed southern parts of the Czech Republic and Slovakia for sand fly presence.

Materials and methods

A field survey to detect sand flies was conducted from July 6 to July 31, 2016 at 41 localities in south-eastern Slovakia, south-western Slovakia (localities from 9 counties) and southern Moravia, Czech Republic (localities from 2 counties) (Table 1). Moreover, collections of insects from past seasons (2012–15) in the same localities in south-eastern Slovakia, as surveyed in 2016 and stored in ethanol, were inspected under a stereomicroscope. Centers for Disease Control (CDC) light traps (John W. Hock) baited with CO2 (dry ice) were placed mostly inside or close to animal shelters and/or organic material both on commercial farms and in private houses where no insecticide spraying was applied. New collection nets from the manufacturer were deployed to exclude possible contamination by sand fly specimens from previous field studies. The traps were set about 2 h before sunset and collected the next morning. Captured insects were killed by freezing in a polystyrene box with dry ice and manually inspected on a sheet of filter paper and under a stereomicroscope.

Table 1.

Localities surveyed during the entomological survey in the Czech Republic and Slovakia.

The sand fly specimen was transferred to 70% ethanol, head and genitalia were slide-mounted using CMCP-9 mounting medium (Polysciences) and the rest of the body was stored in ethanol for molecular analysis. Morphological identification was carried out using published keys and descriptions [4, 10]. Identification was confirmed by a sequencing analysis of the cytochrome oxidase I (COI) gene. Genomic DNA was isolated with a High Pure PCR Template Preparation Kit (Roche). PCR amplification of COI was performed in a 25 µL reaction volume, using the LCO1490/HCO2198 primer pairs and amplification conditions previously described [7]. The amplification products were separated and visualised on 1% agarose gel, purified using a High Pure PCR Product Purification Kit (Roche) and directly sequenced in both directions using the primers used for DNA amplification (ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit). The new COI sequence of the Ph. mascittii specimen from Slovakia (length 620 bp) was deposited in GenBank (Accession Number KX963380). It was blasted against the GenBank database for identification and then aligned and compared with sequences of Ph. mascittii (KX869078, KX981913KX981916) downloaded from GenBank.

Results

Inspection of insects collected in previous seasons in south-eastern Slovakia did not reveal the presence of sand flies. Out of 41 localities surveyed in summer 2016, a single female sand fly was found in one locality, namely Pernek in Slovakia. This village is situated at the western slope of the Small Carpathians, a low mountain range that forms a part of the Western Carpathians mountain system (Fig. 1). The sand fly was trapped in a partly disused barn on a former cattle farm where only about 25 horses are bred at present (Fig. 2).

thumbnail Figure 1.

A map showing the location of Pernek with relation to the nearest previous record of Ph. mascittii in Rohrau, Austria.

thumbnail Figure 2.

A barn on the farm in Pernek, Slovakia where the female Phlebotomus mascittii specimen was collected.

The specimen was identified as a female Phlebotomus mascittii by traditional morphological characters of the pharynx (Fig. 3) and genitalia. The obtained part of the COI gene sequence (GenBank KX963380) was blasted against the GenBank database and identified as Ph. mascittii. A constructed alignment of the sequence of the Slovak specimen with the above-mentioned sequences of Ph. mascittii from Slovenia confirmed the GenBank identification and revealed only a single polymorphic site at position 106.

thumbnail Figure 3.

Pharynx of the examined Ph. mascittii specimen with typical pharyngeal armature.

Discussion

This study presents the first finding of phlebotomine sand fly Phlebotomus mascittii in Slovakia that adds to the several northernmost records of this species in Europe. The fact that it was this particular species is not surprising; it has been assumed that Ph. mascittii has a large range of distribution and it is present throughout most European countries of the Mediterranean basin [9] as well as adjacent areas north of this region, including sporadic findings in Belgium [4], Germany [13, 15], Austria [16] and Hungary [6]. A recent single record in Algeria also suggests its occurrence in North Africa [2]. Other species of the subgenus Transphlebotomus seem to have markedly more restricted distribution. However, a recent description of two new species of this subgenus, Phlebotomus killicki and Ph. anatolicus [8], raised the question of whether the widespread presence of Ph. mascittii may be partly due to these two previously unrecognised species and suggests that exact distribution of species within the genus Transphlebotomus has not yet been delineated unambiguously.

Our finding of Ph. mascittii in southern Slovakia confirms the presence of this species at the northern limit of subgenus Transphlebotomus distribution. This species was previously recorded in neighbouring countries Austria and Hungary. In Hungary, specimens of Ph. mascittii were sporadically recorded in Baranya county at the southern border with Croatia, in Veszprém county close to Lake Balaton and in Pest county in the suburbs of the capital Budapest in 2006–2009 [6]. The latter observation was supported theoretically by climate modelling, suggesting that the peri-urban environment at the outskirts of Budapest would be favourable for this species under certain scenarios [1]. Our survey, however, did not record any sand flies in areas close to the Slovak-Hungarian border. In Austria, Ph. mascittii was first recorded during entomological surveys in Carinthia (2009–2010), the southernmost region of the country neighbouring Slovenia [16] and thus very distant from our positive site in Slovakia. However, a more detailed survey in the following seasons (2012–2013) revealed small but stable populations of Ph. mascittii in localities in Styria, Burgenland and Lower Austria with the northernmost record in the village of Rohrau close to the capital Vienna and Austrian-Slovak borders [17]. This area, called Hundsheimer Berge, is in fact the southernmost extension of the Small Carpathians where our specimen of Ph. mascittii was collected. Future genetic comparison of Austrian and Slovak specimens should reveal whether they belong to one or two closely related populations. Interestingly, the specimen from Slovakia showed almost 100% identity with sequences of P. mascittii specimens from Slovenia in sequences of COI, which is a mitochondrial marker often used in molecular systematics of sand flies [3].

Our knowledge of the biology, ecology and epidemiological significance of Transphlebotomus species in the transmission cycles of leishmaniases is incomplete and sometimes contradictory: while some authors have speculated that Ph. mascittii is autogenous and hence not important for Leishmania transmission [4], others assume that this species readily feeds on dogs and humans and it has been proposed as a potential vector of Leishmania infantum in several small foci of presumably autochthonous canine leishmaniasis in Germany [15]. More importantly, an ITS1 (internal transcribed spacer 1) real-time PCR assay recently revealed one female positive for L. infantum DNA among ten tested ungorged females of Ph. mascittii caught in Austria [18]. However, experimental infections of this species have not yet been studied. It is also unresolved whether Transphlebotomus species share similar habitats with other sand fly species or inhabit special niches. While one of the newly described species, Ph. anatolicus, was collected in typical sand fly habitats near domestic animals [8], other Transphlebotomus species are represented in low numbers in usual sand fly surveys, and Ph. mascittii was recorded mainly from cavernicolous habitats [14]. The disused barn found positive in our study may simulate this type of habitat. Curiously, one Asian elephant (Elephas maximus) belonging to a commercial circus company was also kept close by, although a CDC trap which was placed near to it did not reveal any sand fly specimens.

Our single finding suggests that detailed entomological survey is needed to elucidate the extent of sand fly presence in the region of southern Slovakia, northern Austria and Hungary, as their eventual establishment may have implications concerning possible future transmission of canine or human leishmaniases.

Acknowledgments

The authors would like to thank Eva Bockova, Adela Sarvasova, Tatiana Spitzova, Vera Volfova, David Modrý and Jan Votýpka, for their valuable help during the field work. They would also like to express their appreciation to all farm owners who were willing to allow entomological survey of their premises. The work was carried out under VectorNet, a European network for sharing data on the geographic distribution of arthropod vectors, transmitting human and animal disease agents (Contract OC/EFSA/AHAW/2013/02-FWC1) funded by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC). The study was funded by EurNegVec COST Action TD1303 and COST-CZ LD14076. This research was supported by UNCE (University Research Centre) 204017/2012.

References

  1. Bede-Fazekas Á, Trájer A. 2015. Potential urban distribution of Phlebotomus mascittii Grassi and Phlebotomus neglectus Tonn. (Diptera: Psychodidae) in 2021–50 in Budapest, Hungary. Journal of Vector Borne Diseases, 52, 213–218. [PubMed] [Google Scholar]
  2. Berdjane-Brouk Z, Charrel RN, Bitam I, Hamrioui B, Izri A. 2011. Record of Phlebotomus (Transphlebotomus) mascittii Grassi, 1908 and Phlebotomus (Larroussius) chadlii Rioux, Juminer & Gibily, 1966 female in Algeria. Parasite, 18, 337–339. [CrossRef] [EDP Sciences] [PubMed] [Google Scholar]
  3. Depaquit J. 2014. Molecular systematics applied to Phlebotomine sandflies: review and perspectives. Infections, Genetics and Evolution, 28, 744–756. [CrossRef] [Google Scholar]
  4. Depaquit J, Naucke TJ, Schmitt C, Ferté H, Léger N. 2005. A molecular analysis of the subgenus Transphlebotomus Artemiev, 1984 (Phlebotomus, Diptera, Psychodidae) inferred from ND4 mtDNA with new northern records of Phlebotomus mascittii Grassi, 1908. Parasitology Research, 95, 113–116. [CrossRef] [PubMed] [Google Scholar]
  5. Depaquit J, Grandadam M, Fouque F, Andry PE, Peyrefitte C. 2010. Arthropod-borne viruses transmitted by Phlebotomine sandflies in Europe: a review. Euro Surveillance, 15, 40–47. [Google Scholar]
  6. Farkas R, Tánczos B, Bongiorno G, Maroli M, Dereure J, Ready PD. 2011. First surveys to investigate the presence of canine leishmaniasis and its phlebotomine vectors in Hungary. Vector Borne and Zoonotic Diseases, 11, 823–834. [CrossRef] [Google Scholar]
  7. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299. [PubMed] [Google Scholar]
  8. Kasap OE, Dvorak V, Depaquit J, Alten B, Votypka J, Volf P. 2015. Phylogeography of the subgenus Transphlebotomus Artemiev with description of two new species, Phlebotomus anatolicus n. sp. and Phlebotomus killicki n. sp. Infections, Genetics and Evolution, 34, 467–479. [CrossRef] [Google Scholar]
  9. Léger N, Depaquit J, Ferté H. 2000. Phlebotomine sandflies (Diptera-Psychodidae) of the isle of Cyprus. I – Description of Phlebotomus (Transphlebotomus) economidesi n. sp. Parasite, 7, 135–141. [CrossRef] [EDP Sciences] [PubMed] [Google Scholar]
  10. Lewis DJ. 1982. A taxonomic review of the genus Phlebotomus (Diptera : Psychodidae). Bulletin of the British Museum, Natural History (Entomology), 45, 121–209. [Google Scholar]
  11. Maroli M, Feliciangeli MD, Buchaud L, Charrel RN, Gradoni L. 2013. Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern. Medical and Veterinary Entomology, 27, 123–147. [CrossRef] [PubMed] [Google Scholar]
  12. Medlock JM, Hansford KM, Van Bortel W, Zeller H, Alten B. 2014. A summary of the evidence for the change in European distribution of phlebotomine sand flies (Diptera: Psychodidae) of public health importance. Journal of Vector Ecology, 39, 72–77. [CrossRef] [Google Scholar]
  13. Melaun C, Krüger A, Werblow A, Klimpel S. 2014. New record of the suspected leishmaniasis vector Phlebotomus (Transphlebotomus) mascittii Grassi, 1908 (Diptera: Psychodidae: Phlebotominae)-the northernmost phlebotomine sandfly occurrence in the Palearctic region. Parasitology Research, 113, 2295–2301. [CrossRef] [PubMed] [Google Scholar]
  14. Naucke TJ, Menn B, Massberg D, Lorentz S. 2008. Winter activity of Phlebotomus (Transphlebotomus) mascittii, Grassi 1908 (Diptera: Psychodidae) on the island of Corsica. Parasitology Research, 103, 477–479. [CrossRef] [PubMed] [Google Scholar]
  15. Naucke TJ, Menn B, Massberg D, Lorentz S. 2008. Sandflies and leishmaniasis in Germany. Parasitology Research, 103, S65–S68. [CrossRef] [PubMed] [Google Scholar]
  16. Naucke TJ, Lorentz S, Rauchenwald F, Aspöck H. 2011. Phlebotomus (Transphlebotomus) mascittii Grassi, 1908, in Carinthia: first record of the occurrence of sandflies in Austria (Diptera: Psychodidae: Phlebotominae). Parasitology Research, 109, 1161–1164. [CrossRef] [PubMed] [Google Scholar]
  17. Obwaller A, Poeppl W, Naucke T, Luksch U, Mooseder G, Aspock H, Walochnik J. 2014. Stable populations of sandflies (Phlebotominae) in Eastern Austria: a comparison of the trapping seasons 2012 and 2013. Trends in Entomology, 10, 49–53. [Google Scholar]
  18. Obwaller A, Karakus M, Poeppl W, Töz S, Ozbel Y, Aspock H, Walochnik J. 2016. Could Phlebotomus mascittii play a role as a vector for Leishmania infantum? New data. Parasites & Vectors, 19, 458. [CrossRef] [Google Scholar]
  19. Ready PD. 2010. Leishmaniasis emergence in Europe. Euro Surveillance, 15, 195–206. [Google Scholar]

Cite this article as: Dvorak V, Hlavackova K, Kocisova A & Volf P: First record of Phlebotomus (Transphlebotomus) mascittii in Slovakia. Parasite, 2016, 23, 48.

All Tables

Table 1.

Localities surveyed during the entomological survey in the Czech Republic and Slovakia.

All Figures

thumbnail Figure 1.

A map showing the location of Pernek with relation to the nearest previous record of Ph. mascittii in Rohrau, Austria.

In the text
thumbnail Figure 2.

A barn on the farm in Pernek, Slovakia where the female Phlebotomus mascittii specimen was collected.

In the text
thumbnail Figure 3.

Pharynx of the examined Ph. mascittii specimen with typical pharyngeal armature.

In the text

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