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Table 1.

Binding specificities of lectins used in this study.

Lectin abbreviations Source Concentration (μg/mL) Sugar specificity Inhibitor or eluting sugar*
N-acetylglucosamine
 s-WGA Succinylated-Wheat germ agglutinin 1.0 × 10 −2 GlcNAc 0.2 M GlcNAc
 WGA Wheat germ agglutinin 1.0 × 10 −2 GlcNAc NeuAc, SA 0.2 M GlcNAc
 BSL-II Bandeiraea simplicifolia 4.0 × 10−3 α or β GlcNAc 0.2 M GlcNAc
 DSL Datura stramonium 4.0 × 10−3 (GlcNAc)2–4 0.5 M chitin hydrolysate
 LEL Lycopersicon esculentum 2.0 × 10 −2 (GlcNAc)2–4 0.5 M chitin hydrolysate
 STL Solanum tuberosum 1.0 × 10 −2 (GlcNAc)2–4 0.5 M chitin hydrolysate
Mannose
 ConA Canavalia ensiformis 3.3 × 10−3 αMan, αGlc, 4GlcNAc 0.2 M MeαMan/0.2 M MeαGlc
 LCA Lens culinaris 4.0 × 10−3 αMan, αGlc 4(Fucα1,6)GlcNAc 0.2 M MeαMan /0.2 M MeαGlc
 PSA Pisum sativum 4.0 × 10−3 αMan, αGlc 4(Fucα1,6)GlcNAc 0.2 M MeαMan/0.2 M MeαGlc
Galactose/N-acetylgalactosamine
 RCA120 Ricinus communis 2.0 × 10−3 Gal 0.2 M lactose
 BSL-I Bandeiraea simplicifolia 4.0 × 10−3 αGal, αGalNAc 0.2 M GalNAc
 VVA Vicia villosa 4.0 × 10−3 GalNAc 0.2 M GalNAc
 DBA Dolichos biflorus 1.0 × 10 −2 αGalNAc 0.2 M GalNAc
 SBA Glycine max 1.0 × 10 −2 α > βGalNAc 0.2 M GalNAc
 SJA Sophora japonica 2.0 × 10 −2 βGalNAc 0.2 M GalNAc
 Jacalin Artocarpus integrifolia 5.0 × 10−4 Galβ3GalNAc 0.2 M melibiose
 PNA Arachis hypogaea 4.0 × 10−3 Galβ3GalNAc 0.2 M βGal
 ECL Erythrina cristagalli 2.0 × 10−2 Galβ4GlcNAc 0.2 M lactose
Complex type N-glycans (complex oligosaccharides)
 PHA-E Phaseolus vulgaris 5.0 × 10−3 Galβ4GlcNAcβ 2 Man α6 (GlcNAcβ4)(GlcNAcβ4Manα 3) Manβ4 0.1 M acetic acid
 PHA-L Phaseolus vulgaris 2.5 × 10−3 Galβ4GlcNAcβ6 (GluNAcβ2Man α3) Man α3 0.1 M acetic acid
Fucose
 UEA-I Ulex europaeus 2.0 × 10−2 αFuc 0.1 M L-fucose

Fuc, fucose; gal, galactose; GalNAc, N-acetylgalactosamine; glc, glucose; GlcNAc, N-acetylglucosamine; man, mannose. MeαMan, α-Methylmannoside; MeαGlc, α-Methylglucoside; NeuAc, N-acetylneuraminic acid; SA, sialic acid.

*

The acronyms and lectin sepcificities including sources, preferred sugar specificities and inhibitor were obtained from the data sheet (Vector laboratory) and a previous paper [7].

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