Open Access
Table 1.
Binding specificities of lectins used in this study.
Lectin abbreviations | Source | Concentration (μg/mL) | Sugar specificity | Inhibitor or eluting sugar* |
---|---|---|---|---|
N-acetylglucosamine | ||||
s-WGA | Succinylated-Wheat germ agglutinin | 1.0 × 10 −2 | GlcNAc | 0.2 M GlcNAc |
WGA | Wheat germ agglutinin | 1.0 × 10 −2 | GlcNAc NeuAc, SA | 0.2 M GlcNAc |
BSL-II | Bandeiraea simplicifolia | 4.0 × 10−3 | α or β GlcNAc | 0.2 M GlcNAc |
DSL | Datura stramonium | 4.0 × 10−3 | (GlcNAc)2–4 | 0.5 M chitin hydrolysate |
LEL | Lycopersicon esculentum | 2.0 × 10 −2 | (GlcNAc)2–4 | 0.5 M chitin hydrolysate |
STL | Solanum tuberosum | 1.0 × 10 −2 | (GlcNAc)2–4 | 0.5 M chitin hydrolysate |
Mannose | ||||
ConA | Canavalia ensiformis | 3.3 × 10−3 | αMan, αGlc, 4GlcNAc | 0.2 M MeαMan/0.2 M MeαGlc |
LCA | Lens culinaris | 4.0 × 10−3 | αMan, αGlc 4(Fucα1,6)GlcNAc | 0.2 M MeαMan /0.2 M MeαGlc |
PSA | Pisum sativum | 4.0 × 10−3 | αMan, αGlc 4(Fucα1,6)GlcNAc | 0.2 M MeαMan/0.2 M MeαGlc |
Galactose/N-acetylgalactosamine | ||||
RCA120 | Ricinus communis | 2.0 × 10−3 | Gal | 0.2 M lactose |
BSL-I | Bandeiraea simplicifolia | 4.0 × 10−3 | αGal, αGalNAc | 0.2 M GalNAc |
VVA | Vicia villosa | 4.0 × 10−3 | GalNAc | 0.2 M GalNAc |
DBA | Dolichos biflorus | 1.0 × 10 −2 | αGalNAc | 0.2 M GalNAc |
SBA | Glycine max | 1.0 × 10 −2 | α > βGalNAc | 0.2 M GalNAc |
SJA | Sophora japonica | 2.0 × 10 −2 | βGalNAc | 0.2 M GalNAc |
Jacalin | Artocarpus integrifolia | 5.0 × 10−4 | Galβ3GalNAc | 0.2 M melibiose |
PNA | Arachis hypogaea | 4.0 × 10−3 | Galβ3GalNAc | 0.2 M βGal |
ECL | Erythrina cristagalli | 2.0 × 10−2 | Galβ4GlcNAc | 0.2 M lactose |
Complex type N-glycans (complex oligosaccharides) | ||||
PHA-E | Phaseolus vulgaris | 5.0 × 10−3 | Galβ4GlcNAcβ 2 Man α6 (GlcNAcβ4)(GlcNAcβ4Manα 3) Manβ4 | 0.1 M acetic acid |
PHA-L | Phaseolus vulgaris | 2.5 × 10−3 | Galβ4GlcNAcβ6 (GluNAcβ2Man α3) Man α3 | 0.1 M acetic acid |
Fucose | ||||
UEA-I | Ulex europaeus | 2.0 × 10−2 | αFuc | 0.1 M L-fucose |
Fuc, fucose; gal, galactose; GalNAc, N-acetylgalactosamine; glc, glucose; GlcNAc, N-acetylglucosamine; man, mannose. MeαMan, α-Methylmannoside; MeαGlc, α-Methylglucoside; NeuAc, N-acetylneuraminic acid; SA, sialic acid.
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