Issue |
Parasite
Volume 23, 2016
|
|
---|---|---|
Article Number | 21 | |
Number of page(s) | 8 | |
DOI | https://doi.org/10.1051/parasite/2016021 | |
Published online | 11 May 2016 |
Article
Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
Histochimie des lectines du muscle du cardeau hirame (Paralichthys olivaceus) infecté par Kudoa septempunctata génotype ST3
1
College of Veterinary Medicine, Jeju National University, Jeju
63243, Republic of Korea
2
Incheon International Airport Regional Office, National Fishery Products Quality Management Service, Ministry of Oceans and Fisheries, Incheon
22382, Republic of Korea
3
Ocean and Fisheries Research Institute, Jeju Special Self-Governing Province, Pyoseon-myeon, Segwipo-si, Jeju
63629, Republic of Korea
4
College of Medicine, Jeju National University, Jeju
63243, Republic of Korea
* Corresponding author: shint@jejunu.ac.kr
a Co-corresponding author: healthy@jejunu.ac.kr
Received:
30
January
2016
Accepted:
23
April
2016
The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3.
Résumé
La localisation des terminaux de carbohydrates dans le muscle du cardeau hirame (Paralichthys olivaceus) infecté par Kudoa septempunctata génotype ST3 a été étudiée en utilisant l’histochimie des lectines, afin de déterminer les types de résidus d’hydrate de carbone exprimés dans les spores de Kudoa. Vingt-et-une lectines ont été examinées, à savoir N-acétylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acétylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, jacaline, PNA, ECL), glycanes complexes de type N (PHA-E et PHA-l) et fucose (UEA-I). Les spores enveloppées dans une membrane plasmodiale ont été marquées par la majorité de ces lectines, à l’exception de LCA, PSA, PNA et PHA-l. Quatre lectines (RCA 120, BSL-I, DBA et SJA), appartenant au groupe galactose/N-acétylgalactosamine, ont marqué seulement les spores mais pas la membrane plasmodiale. Ceci est la première confirmation que divers résidus de sucre sont présents dans les spores et les membranes plasmodiales de K. septempunctata ST3.
Key words: Kudoa septempunctata / Lectin / Myxozoa / Olive flounder
© J. Kang et al., published by EDP Sciences, 2016
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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