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Figure 3.


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Expression of GST-PP5 in BL-21 cells. Expression of GST-PP5 as detected by Western blotting using an anti-GST antibody (left panel) induced with 0.1 mM IPTG or uninduced. Right panel, polyclonal anti-PP5 antibody. Lanes are representative of the elution process: total protein lysate (lane 1), flow through washes (lanes 2 and 3) and GST-PP5 protein eluate (lane 4). The molecular weight of standard proteins is indicated in kDa.

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