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Immunodetection of EhRAD54, EhRAD51, and EhBLM. (A) Localization of EhRAD54-pepA and EhBLM-pepB peptides in the 3D model of each protein. (B) Western blot assays of cytoplasmic (CE) and nuclear (NE) extracts from trophozoites without irradiation (No UV-C) or at 5 min or 30 min after DNA damage. The name of each inmmunodetected protein is indicated at the right. Actin was used as control. (C) Densitometry analysis of bands revealed in (B). Pixels corresponding to cytoplasmic actin were taken as 100% at each time after DNA damage, and used to normalize data. The relative expression of each protein is expressed using the protein amount before UV-C exposure as a reference. Results in (B) and (C) are representative of three replicates.
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