Issue |
Parasite
Volume 30, 2023
|
|
---|---|---|
Article Number | 58 | |
Number of page(s) | 13 | |
DOI | https://doi.org/10.1051/parasite/2023057 | |
Published online | 12 December 2023 |
Research Article
Microbial diversity of ticks and a novel typhus group Rickettsia species (Rickettsiales bacterium Ac37b) in Inner Mongolia, China
Diversité microbienne des tiques et nouvelle espèce de Rickettsia du groupe du typhus (bactérie Rickettsiales Ac37b) en Mongolie intérieure, Chine
1
Graduate School, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia, China
2
School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia, China
3
First Hospital of Jilin University, Changchun 130021, China
4
Hulunbuir Mental Health Center, Hulunbuir 022150, Inner Mongolia, China
5
Beijing Guoke Biotechnology Co., Ltd, 102200 Beijing, China
6
Department of Infection Control, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010000, China
7
Inner Mongolia Academy of Agricultural & Animal Husbandry Science, Hohhot 010031, Inner Mongolia, China
* Corresponding authors: 1184474898@qq.com; fushao1234@126.com; Gaoruijuan01@126.com; 1420352185@qq.com
Received:
1
August
2023
Accepted:
20
November
2023
Ticks can carry multiple pathogens, and Inner Mongolia’s animal husbandry provides excellent environmental conditions for ticks. This study characterized the microbiome of ticks from different geographical locations in Inner Mongolia; 905 Dermacentor nuttalli and 36 Ixodes persulcatus were collected from sheep in three main pasture areas and from bushes within the forested area. Mixed DNA samples were prepared from three specimens from each region and tick species. Microbial diversity was analyzed by 16S rRNA sequencing, and α and β diversity were determined. The predominant bacterial genera were Rickettsia (54.60%), including Rickettsiales bacterium Ac37b (19.33%) and other Rickettsia (35.27%), Arsenophonus (11.21%), Candidatus Lariskella (10.84%), and Acinetobacter (7.17%). Rickettsia bellii was identified in I. persulcatus, while Rickettsiales bacterium Ac37b was found in D. nuttalli from Ordos and Chifeng. Potential Rickettsia and Anaplasma coinfections were observed in the Ordos region. Tick microbial diversity analysis in Inner Mongolia suggests that sheep at the sampling sites were exposed to multiple pathogens.
Résumé
Les tiques peuvent être porteuses de plusieurs agents pathogènes et l’élevage en Mongolie intérieure offre d’excellentes conditions environnementales pour les tiques. Cette étude a caractérisé le microbiome des tiques de différentes zones géographiques de Mongolie intérieure; 905 Dermacentor nuttalli et 36 Ixodes persulcatus ont été collectés sur des moutons dans trois principales zones de pâturage et dans des buissons de la zone forestière. Des échantillons d’ADN mixtes ont été préparés à partir de trois spécimens de chaque région et espèce de tique. La diversité microbienne a été analysée par séquençage de l’ARNr 16S et la diversité α et β a été déterminée. Les genres bactériens prédominants étaient les Rickettsia (54,60 %), dont la bactérie Rickettsiales Ac37b (19,33 %) et d’autres Rickettsia (35,27 %), Arsenophonus (11,21 %), Candidatus Lariskella (10,84 %) et Acinetobacter (7,17 %). Rickettsia bellii a été identifiée chez I. persulcatus, tandis que la bactérie Rickettsiales Ac37b a été trouvée chez D. nuttalli d’Ordos et Chifeng. Des co-infections potentielles à Rickettsia et Anaplasma ont été observées dans la région d’Ordos. L’analyse de la diversité microbienne des tiques en Mongolie intérieure montre que les moutons présents sur les sites d’échantillonnage sont exposés à plusieurs agents pathogènes.
Key words: Inner Mongolia / Dermacentor nuttalli / Ixodes persulcatus / Microbial diversity / Rickettsiales bacterium Ac37b
© S. Su et al., published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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