Issue |
Parasite
Volume 20, 2013
|
|
---|---|---|
Article Number | 8 | |
Number of page(s) | 7 | |
DOI | https://doi.org/10.1051/parasite/2013008 | |
Published online | 12 March 2013 |
Research Article
Molecular identification of Giardia and Cryptosporidium from dogs and cats
Identification moléculaire de Giardia et Cryptosporidium chez les chiens et chats
1
Medical and Molecular Parasitology Laboratory, Medical School, Centre of Anatomy, Institute II, University of Cologne, Joseph-Stelzmann Str. 9, D-50931 Cologne, Germany
2
Centre of Dental Medicine, Department of Operative Dentistry and Periodontology, University of Cologne, Kerpenerstr. 32, D-50931 Cologne, Germany
3
Idexx Vet Med Lab, Moerikestrasse 28/3, D-71636 Ludwigsburg, Germany
* Corresponding author: isaia.sotiriadou@uk-koeln.de
Received:
10
January
2012
Revised:
5
February
2013
Accepted:
22
February
2013
The aim of the present study was to diagnose the presence of Giardia cysts and Cryptosporidium oocysts in household animals using nested polymerase chain reaction (PCR) and sequence analysis. One hundred faecal samples obtained from 81 dogs and 19 cats were investigated. The Cryptosporidium genotypes were determined by sequencing a fragment of the small subunit (SSU) rRNA gene, while the Giardia Assemblages were determined through analysis of the glutamate dehydrogenase (GDH) locus. Isolates from five dogs and two cats were positive by PCR for the presence of Giardia, and their sequences matched the zoonotic Assemblage A of Giardia. Cryptosporidium spp. isolated from one dog and one cat were both found to be C. parvum. One dog isolate harboured a mixed infection of C. parvum and Giardia Assemblage A. These findings support the growing evidence that household animals are potential reservoirs of the zoonotic pathogens Giardia spp. and Cryptosporidium spp. for infections in humans.
Résumé
Le but de cette étude était de diagnostiquer la présence de kystes de Giardia et d’oocystes de Cryptosporidium chez des animaux de compagnie en utilisant la réaction en chaîne de la polymérase (PCR) et l’analyse des séquences. Cent échantillons de fèces obtenus de 81 chiens et 19 chats ont été étudiés. Les génotypes de Cryptosporidium ont été déterminés en séquençant un fragment de la petite unité (SSU) du gène rRNA, et les Assemblages de Giardia ont été déterminés par une analyse du locus de la glutamate déshydrogénase (GDH). Des isolats de cinq chiens et de deux chats ont été positifs par PCR pour la présence de Giardia, et leurs séquences correspondaient à l’Assemblage A de Giardia. Les Cryptosporidium isolées d’un chien et d’un chat appartenaient tous deux à l’espèce C. parvum. Un chien avait une infection mixte par C. parvum et Giardia Assemblage A. Ces résultats confirment que les animaux de compagnie sont des réservoirs potentiels des pathogènes zoonotiques Giardia spp. et Cryptosporidium pour des infections humaines.
Key words: Cryptosporidium parvum / Giardia / Assemblages / genotypes / zoonosis / Germany
© I. Sotiriadou et al., published by EDP Sciences, 2013
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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