Issue |
Parasite
Volume 13, Number 1, March 2006
|
|
---|---|---|
Page(s) | 51 - 58 | |
DOI | https://doi.org/10.1051/parasite/2006131051 | |
Published online | 15 March 2006 |
Mémoire
Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar
Les effets d’accélération de croissance des protéines fer-soufre dans la culture axénique d’Entamoeba dispar
1
Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan
2
Department of Medical Parasitology and Mycology, School of Medicine Shaheed University of Medical Sciences, Eveen, Teheran 19395, Iran
* Correspondence: Seiki Kobayashi, PhD., Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. Tel.: +81-3-5363-3761 – Fax: +81-3-3353-5958. E-mail: skobaya@sc.itc.keio.ac.jp
Received:
14
June
2005
Accepted:
4
November
2005
A growth-promoting factor (GPF) that promotes the growth of Entamoeba dispar under axenic culture conditions was found in fractions of mitochondria (Mt), hydrogenosomes (Hg) and chloroplasts (Cp) obtained from cells of six different protozoan, mammalian and plant species. We were able to extract the GPF from the Cp-rich leaf cells of a plant (spiderwort: Commelina communis L.) in an acetone-soluble fraction as a complex of chlorophyll with low molecular weight proteins (molecular weight [MW] approximately 4,600). We also found that on treatment with 0.6 % complexes of 2-mercapthoethanol (2ME), complexes of chlorophyll-a with iron-sulphur (Fe-S) proteins (e.g., ferredoxins [Fd] from spinach and Clostridium pasteurianum) and noncomplex rubredoxin (Rd) from C. pasteurianum have a growth-promoting effect on E. dispar. These findings suggest that E. dispar may lack a sufficient quantity of some essential components of Fe-S proteins, such as Fe-S center.
Résumé
Des facteurs d’accélération de croissance (Growth promoting factor: GPF) favorisant le développement d’Entamoeba dispar ont été détectés dans la composition de la mitochondrie, de l’hydrogénosome et du chloroplaste isolés à partir de six sortes de cellules issues de protozoaires, mammifères et plantes. De plus, les GPF de cellules de mésophile contenant une grande quantité de chloroplaste végétal (Commelina communis L.) ont pu être extraits comme une substance composée de protéines de faible masse moléculaire (≈ 4600) et de chloroplastes dans la composition acétone-soluble. À partir de ces résultats, nous avons préalablement pu découvrir des effets d’accélération de croissance dans le corps composé de protéines fer-soufre (épinard et ferrédoxine de Clostridium pasteurianum) et de chlorophylle-a traité au 2-mercaptoéthanol 0,6 % et du corps simple rubrédoxine de C. pasteurianum. Ces observations ont suggéré qu’une composante essentielle formant la protéine fer-soufre d’E. dispar (comme noyau fer-soufre) semblait être insuffisante.
Key words: growth / mitochondria / hydrogenosomes / chloroplasts / iron-sulphur protein / Entamoeba dispar
Mots clés : croissance / mitochondrie / hydrogénosome / chloroplaste / protéine fer-soufre / Entamoeba dispar
© PRINCEPS Editions, Paris, 2006, transferred to Société Française de Parasitologie
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