Characterization of chronic neurotoxocarosis in APP/PS1 murine model. (A) Relative weight changes differ between infected and control groups and also between infected WT and APP/PS1 mice at 16 WPI. The graph contains only data from mice that did not undergo behavioral testing. (B) Levels of serum anti-T. canis IgG antibodies were elevated both in infected WT and APP/PS1 mice, the latter showing even significantly higher values. (C) T. canis larvae mostly preferred the hemispheres in both groups. Interestingly, more larvae were found in the APP/PS1 group. The values show larval burden in half of the freshly harvested organ as the other half was used for Aβ measurement (see next). (D) Chronic neurotoxocarosis did not alter Aβ production in the hemispheres, higher levels of Aβ were observed in the APP/PS1 groups as expected. Individual data are shown along with the group median. For statistical analysis, a mixed-effects model (A) or 2-way ANOVA (B–D) followed by Holm-Šidák’s multiple comparisons test were used. Significant differences among the infected groups are indicated by asterisks (*p ≤ 0.05, ***p ≤ 0.001). Aβ, amyloid-β; CNS, central nervous system; Ctrl, control (uninfected) group; OD, optical density; WPI, weeks post infection.