Viability assessment of T. canis larvae after in vitro treatment with recombinant Aβ_1-42. After one week of treatment, neither morphology (A) or staining with vital (fluorescein diacetate; FDA) and non-vital (propidium iodide; PI) dyes (B) showed alterations in larval viability. Moreover, the 7-day treatment did not disrupt the surface of larvae as revealed by scanning electron microscopy (C). For statistical analysis, 1-way ANOVA followed by Dunnett’s multiple comparisons test were used to compare the treated groups to the respective negative control. Significant differences are indicated by asterisks (****p ≤ 0.0001). Scale bars: 10 μm.