Characterization of chronic neurotoxocarosis in BALB/c murine model. (A) Relative weight changes did not differ between infected and control groups. Crosses (“+”) indicate mice that showed body weight drop; two of them died 13 WPI. The graph contains mice pooled from two experiments (harvest 8 and 16 WPI). (B) Levels of serum anti-T. canis IgG antibodies were significantly elevated in infected mice at both time points. (C) T. canis larvae accumulated mostly in hemispheres at both time points. Overall, more larvae were found in the group sacrificed 8 WPI. The values show larval burden in half of the freshly harvested organ as the other half was used for Aβ measurement (see next). (D) Chronic neurotoxocarosis did not alter Aβ production in the hemispheres. Individual data are shown along with the group median. For statistical analysis, a mixed-effects model (A) or 2-way ANOVA (B–D) followed by Holm-Šidák’s multiple comparisons test were used. Significant differences among the infected groups are indicated by asterisks (*p ≤ 0.05, ****p ≤ 0.0001). Aβ, amyloid-β; CNS, central nervous system; Ctrl, control (uninfected) group; OD, optical density; WPI, weeks post infection.