Number and scoring of N. brasiliensis proteins fulfilling the criteria of the applied reverse vaccinology approach. The figure presents the number of N. brasiliensis proteins: (A) with homologs in N. americanus based on different sequence length coverage thresholds (60%, 70%, or 80%) and identity threshold (0%, 40%, 50%, 60%, 70%, or 80%) combinations; (B) with homologs in H. sapiens and M. musculus reference proteomes at distinct identity cut-offs (0%–60% homology); (C) categorized by cellular locations of immunological interest; (D) with homologs expressed in the gut of the related hookworm A. ceylanicum; (E) with protease functional homologs (see Materials and methods for group details); (F) binned according to the number of residues within predicted B-cell epitopes; (G) binned according to the number of HLA class-II supertypes with at least one predicted epitope.