Expression of DC surface marker molecules induced by ES antigen, detected by flow cytometry. DCs were cultured in standard RPMI 1640 medium or stimulated with ES antigen or LPS (positive control) for 24 h. (A–D) Expression levels of CD40, MHC-II, CD80, and CD86 were analyzed by flow cytometry. (E) Ratios of CD40, MHC-II, CD80, and CD86 in different groups. *p < 0.05, **p < 0.01, ***p < 0.001.