Open Access

Table 1

PCR primers used in the genetic characterization of the hypothetical protein 1 (hp1) and tubulin 1 (tub1) loci in Enterocytozoon bieneusi.

Locus Gene tag Description Primers (5′ 3′) Annealing temp (°C) Expected product size (bp)
hp1 EBI_21704 Hypothetical protein 1 F1, TTGTGGATTTCCTGTCAAAGA 55 775
R1, TAAGGATGTAGTTACAAATGGAT
F2, ATGATGTTATTTGCGATGATGT 55 675
R2, GTTCTAATTTTAATGGAACAAATGGA
tub1 EBI_21729 Tubulin 1 F1, CTGCAACGCGTTGTTCTT 55 811
R1, CGTAATTTAACATATGCAACCTCA
F2, AGTATTCACTCAGAAGCGATT 55 746
R2, TTAGTGGTTTAACTTTAGCAACATT

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