Figure 1

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Efficiency of nested PCR analysis of the hp1 and tub1 loci from Enterocytozoon bieneusi. The amplification efficiency of Group 1 (65.9%), Group 2 (65.4%), Group 3 (60.0%), Group 4 (100.0%), Group 5 (55.6%), Group 6 (71.4%), and Group 10 (80.0%) at the hp1 locus were all greater than 50%. The amplification efficiency of Group 1 (69.8%), Group 2 (76.6%), Group 4 (100.0%), Group 5 (62.5%), and Group 10 (100.0%) at the tub1 locus were all greater than 50%. The number shown at the top of the bar chart indicates the size of samples analyzed.
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