Differentially expressed genes (DEGs) of S. japonicum after ds-GFP RNAi in vivo. Adult male and female S. japonicum were harvested at 28 dpi and RNA-Seq was performed on replicate samples of dsGFP.F (female S. japonicum worms treated with ds-GFP), dsGFP.M (male S. japonicum worms treated with ds-GFP), NACL.F (female S. japonicum worms treated with NaCl), and NACL.M (male S. japonicum worms treated with NaCl). (A) PCA results. Each symbol indicates an individual sample. (B) Pearson’s correlation analysis between samples. (C) Volcano plots showing differentially expressed genes (DEGs) in ds-GFP.F compared to NaCl.F. (D) Volcano plots showing differentially expressed genes (DEGs) in ds-GFP.M compared to NaCl.M.