The growth and development of S. japonicum remained largely unaffected by ds-GFP. Adult male and female S. japonicum were harvested at 28 dpi. (A) Representative of female and male worm body-length between ds-GFP and NaCl. (B) Female worm body-length measurements. ns p > 0.05 by unpaired t-test; n > 30. (C) Male worm body-length measurements. ns p > 0.05 by unpaired t-test; n > 30. (D) Number of female and male worms per mouse. ns p > 0.05 by unpaired t-test; n = 4. (E) Number of eggs from ds-GFP or NaCl treated mice. EPG: eggs per gram; EPGF: egg counts per gram of faeces. ns p > 0.05 by unpaired t-test; n = 4. (F) Histological assessment of mouse liver by staining. (G) Histological assessment of mouse liver by Masson staining. Each group had four biological replicates, the images shown were representative experimental results of one mouse. Error bars represent the SEM.