Proportion of immune cells in mice spleen not affected by ds-GFP. Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized 28 days after NaCl or ds-GFP treatment. Lymphocytes from spleen of mice were pooled and nylon-wool purified and CD11b and CD3 cells were stained for flow cytometric analysis. (A) Representative flow plots of CD11b cells in spleen at 28 days after NaCl or ds-GFP treatment. (B) Frequency of CD11b cells. (C) Representative flow plots of CD3 T cells in spleen at 28 days after NaCl or ds-GFP treatment. (D) Frequency of CD3 T cells. ns p > 0.05 by unpaired t-test; n = 4. Error bars represent the SEM.