Quantification of TsGDH expression in diverse Trichinella spiralis stages performed by qPCR and Western blotting. A: qPCR assay of TsGDH mRNA expression in diverse T. spiralis stages. The TsGDH transcription level was assessed by the 2^{− ΔΔCt} method. GAPDH served as an internal control. B: Western blot analysis was performed to determine the TsGDH protein expression level in various T. spiralis stages. Transcription and expression levels of TsGDH at 3 and 6 d AWs was obviously higher than other worm stages. *p < 0.01 compared with the ML stage.