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Figure 3

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Expression and identification of rTsGDH. A: SDS-PAGE analysis of rTsGDH. Lane M: protein marker; Lane 1: BL21 carrying pQE-80L/TsGDH before induction. Lane 2: BL21 carrying pQE-80L/TsGDH after being induced with 0.5 mM IPTG at 25 °C for 12 h. Lane 3: purified rTsGDH indicated by a black arrow. B: Western blotting analysis of rTsGDH antigenicity. Lane 1: BL21 carrying pQE-80L/TsGDH before induction was not recognized by infection serum. Lane 2: BL21 carrying pQE-80L/TsGDH after induction was not recognized by infection serum. The purified rTsGDH was recognized by anti-rTsGDH serum (Lane 4) and anti-his tag McAb (Lane 5), but not by infection serum (Lane 3) and normal serum (Lane 6). C: SDS-PAGE analysis of ES proteins of T. spiralis ML (Lane 1), IIL (Lane 2) and 6 d AW (Lane 3). D: Western blotting analysis of T. spiralis worm ES proteins. The ML ES proteins were recognized by infection serum (Lane 1), but not by anti-rTsGDH serum (Lane 2) and normal serum (Lane 3). The ES proteins of IIL (Lane 4) and 6 d AW (Lane 5) were not recognized by anti-rTsGDH serum.

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