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Figure 12

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dsRNA-TsGDH inhibition of Trichinella spiralis larval invasion and molting in vitro. A: T. spiralis larva damage and invasion into the Caco-2 cell monolayer observed under a microscope. The migratory larva extended and migrated in the Caco-2 monolayer. The red arrow shows the larval migratory trace into the Caco-2 monolayer. The non-invaded larva was coiled. Scale bar: 100 μm. B: Inhibitory effect of dsRNA-TsGDH on T. spiralis larva invasion. C: Larval molting in different groups. No obvious molting traces in larval tail were observed in the dsRNA-TsGDH group, while obvious molting sheaths in the larval head and tail were observed in the GFP and PBS groups. D: dsRNA-TsGDH reduced the molting rate of the larvae. The area in the red box was enlarged for observation. *p < 0.05 relative to the PBS group.

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