Morphology, complete mitochondrial genome, and molecular phylogeny of Rhabdias macrocephalum n. sp. (Nematoda: Rhabdiasidae) from Diploderma splendidum (Reptilia: Agamidae)

Open Access

Table 1

The primers and cycling conditions for amplifying different target regions by polymerase chain reaction (PCR) in the present study.

Primer	Sequence 5′-3′	Cycling condition	Source
ITS regions (ITS-1 + 5.8S + ITS-2)	#93: 5′-TTGAACCGGGTAAAAGTCG-3′	94 °C for 3 min	[7]
	#93: 5′-TTGAACCGGGTAAAAGTCG-3′	94 °C for 30 s
	#94: 5′-TTAGTTTCTTTTCCTCCGCT-3′	54 °C for 30 s
		72 °C for 60 s (35 cycles)
		72 °C for 7 min
28S	#500: 5′-ACTTTGAAGAGAGAGTTCAAGAG-3′	94 °C for 3 min	[7]
	#500: 5′-ACTTTGAAGAGAGAGTTCAAGAG-3′	94 °C for 30 s
	#501: 5′-TCGGAAGGAACCAGCTACTA-3′	54 °C for 30 s
		72 °C for 60 s (35 cycles)
		72 °C for 7 min
cox1	LCO1490: 5′-GGTCAACAAATCATAAAGATATTGG-3′	95 °C for 3 min	[8]
	LCO1490: 5′-GGTCAACAAATCATAAAGATATTGG-3′	95 °C for 30 s
	HCO2198: 5′-TAAACTTCAGGGTGACCAAAAAATCA-3′	50 °C for 30 s
		72 °C for 90 s (45 cycles)
		72 °C for 10 min
12S	12S-F: 5′-GTTCCAGAATAATCGGCTA-3′	94 °C for 3 min	[6]
	12S-F: 5′-GTTCCAGAATAATCGGCTA-3′	94 °C for 45 s
	12S-R: 5′-ATTGACGGATG(AG)TTTGTACC-3′	48 °C for 45 s
		72 °C for 1 min (35 cycles)
		72 °C for 5 min
cox2	cox2-F: 5′-AGGTATAAAACTGTGATTTGCACCA-3′	95 °C for 15 min	Present study
	cox2-F: 5′-AGGTATAAAACTGTGATTTGCACCA-3′	95 °C for 45 s
	cox2-R: 5′-TGTTTTCTGGCAGTTTGTTTTCT-3′	48 °C for 45 s
		72 °C for 1 min (35 cycles)
		72 °C for 5 min

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