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Material and methods. (A) Bed bug (Cimex lectularius). (B) Bed bug on folded paper support. (C) Bed bugs and paper supports in plastic containers. (D) Incubator at 24 ± 1 °C, with 56% relative humidity photoperiod 12–12 h. (E) Blood feedings twice a week on “Hemotek” feeding system. (F) Dust testing equipment: Petri dish, cotton fabric at the bottom, capsule filled in with dust with standardized 0.21 mL volume. (G) Experimental conditions: the dust is distributed evenly, and bed bugs are placed on the dusted support. (H) Repellent experiment: bed bugs were placed on the central axis between the dusted and non-dusted areas. (I) A dead bed bug after exposure to SD.
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