TBR1/2 PCR identification of Trypanosoma brucei s.l in tsetse fly saliva showing 177 bp DNA satellite repeat specific for T. brucei s.l – numbers correspond to those assigned to the flies. M: 100 bp DNA size marker; Tex: DNA extraction negative control; T−: PCR-negative control; T+: positive control; (1): Strong PCR signal; (2): Medium PCR signal; (3): Weak PCR signal; (4): Negative PCR signal.