mRNA expression level of M1/M2 genes in RAW264.7 macrophages. The mRNA expression of M1/M2 cytokines in RAW264.7 macrophages treated with rTsgal or GM was analyzed by qPCR. After treatment with 20 μg/mL rTsgal or 1.6% GM at 37 °C for 24 h, the transcription level of M1 cytokines (IL-1β, IL-6 and TNF-α) was clearly increased in RAW264.7 macrophages, whereas the transcription level of M2 cytokines (IL-10, TGF-β) and surface marker CD206 was not evidently changed. The mRNA expression levels of the cytokines were calculated with the Ct ^(2−ΔΔCt) method. GAPDH was utilized as an internal control. *p < 0.0001 compared to the DMEM group.