Expression level of M1/M2 marker protein in RAW264.7 cells (A) and murine peritoneal macrophages (B). The expression of iNOS and Arg1 in macrophages was analyzed on Western blotting after the cells were incubated with 20 μg/mL rTsgal or 1.6% GM at 37 °C for 24 h. Tubulin served as the internal control. Each experiment was performed in triplicate. Arg1 expressed in RAW264.7 macrophages had a monomer band (A); whereas Arg1 expressed in murine peritoneal macrophages had a dimer band (B). *p < 0.0001 compared to the DMEM group.