Killing effects of ADCC on the NBL. Murine peritoneal macrophages were pre-incubated with GM and rTsgal, 50 NBL were added into the medium containing infection serum and incubated at 37 °C for 72 h. After the NBL were cultured with various groups of macrophages and infection serum, larval viability was determined based on larval morphology and activity. Cytotoxicity was assessed as the percentage of dead NBL or NBL with adherent macrophages to the number of total larvae observed in each assay. A: The killing effect of ADCC on NBL was observed under microscopy at various cultivation times. B: The cytotoxicity of various groups of murine peritoneal macrophages on NBL at 72 h after cultivation. C: The cytotoxicity had an elevating trend with prolongation of culture time. *p < 0.05 compared to the DMEM group. Scale bars: 200 μm.