Expression of M1/M2 markers in mouse peritoneal macrophages determined by flow cytometry. The peritoneal macrophages with F4/80⁺CD11b⁺CD86⁺ phenotype were identified as M1 macrophages, and those with a F4/80⁺CD11b⁺CD206⁺ phenotype were designated as M2 macrophages. A: Gating strategy of macrophages. The cell population was analyzed in the P1 gate, and single cell in the P2 was circled using forward scatter-area (FSC-A) and forward scatter-height (FSC-H). Macrophages were identified using F4/80 and CD11b antibodies in the P3. B: Black box displays M1 cells (F4/80⁺CD11b⁺CD86⁺). C: The percentage of CD86 positive cells is shown in the bar graph. D: Black box displays the F4/80⁺CD11b⁺CD206⁺ M2 macrophages. E: The percentage of CD206 positive cells is shown in the bar graph. *p < 0.01 compared to the DMEM group.