Tsgal transcription and expression levels in diverse T. spiralis phases. A: qPCR analysis of Tsgal transcription in different T. spiralis phases. The relative Tsgal mRNA expression level at IIL and 6 d AW phases was evidently higher than the other worm stages. B: Western blot analysis of the Tsgal expression level in different T. spiralis phases. Tsgal expression level at IIL and 6 d AW phases was also significantly higher than other worm phases. Asterisks indicate a statistically significant difference compared to the ML stage (*p < 0.05).