Open Access
Table 1
Gene symbols, flybase ID and primer sequences used to assess Turandot (tot) gene expression with RT-qPCR. Ribosomal protein genes (Rps20, Rpl32, and RpL11) were used as housekeeping genes.
| Gene symbol | Flybase ID | Forward Primer (3′ 5′) | Reverse Primer(3′ 5′) | Fragment length (bp) |
|---|---|---|---|---|
| TotA | FBgn0028396 | TTCCGACGAAGATCGTGAGG | CTGGGTGCTATTGATTTTGGAGT | 91 |
| TotC | FBgn0044812 | ATTCTGACGAGGAAAGGGAATC | CTTGGGTTCGATTGATCTTCGAT | 93 |
| TotX | FBgn0044810 | GCAGCTTGCTAATATGCGTGT | TCGGATAGAGGAACATCTGTAGG | 199 |
| TotM | FBgn0031701 | TCACAGAAAAACAGCGCCTAT | ATCGTAGAAAGTGACCAGGCT | 98 |
| RpS20 | FBgn0019936 | TGGTGATGCGAAGGGTCTTG | CCGCATCACCCTGACATCC | 134 |
| RpL32 | FBgn0002626 | TATTCCGACCACGTTACAAGAAC | GCTTCAAGGGACAGTATCTGATG | 306 |
| RpL11 | FBgn0013325 | GTATTCGCCGTAACGAGAAGAT | GATGCCGAAACCGAAGTTGC | 146 |
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