Phylogenetic relationships of E. bieneusi genotypes in this study, with reference sequences downloaded from GenBank based on the sequence analysis of the ITS locus using Maximum Likelihood analysis with the General Time Reversible model. Red and blue filled circles before the bold sample names represent novel and known genotypes identified in the present study, respectively. Bootstrap values (> 50) are indicated above the nodes. Scale bar indicates 0.1 nucleotide substitutions/site. Genotype SW3 from stormwater (KF591679.1) is used as the outgroup.