Open Access
Table 1
qPCR primers for key components in the TLR4 signaling pathway, cytokines and egr-miR-71.
| Gene symbol | Accession number1 | Gene name | Sequence (5′–3′) | Size (bp) |
|---|---|---|---|---|
| GAPDH | NM_001190390.1 | Glyceraldehyde-3-phosphate dehydrogenase | F: CTAGGCTACACTGAGGAC | 74 |
| R: CAGCATCGAAGGTAGAAG | ||||
| TLR4 | NM_001135930.1 | Toll like receptor 4 | F: GTATCTCTAGATGACTTCCC | 11 |
| R: CAGGTTGGGAAGGTCAGAAA | 7 | |||
| CD14 | NM_001077209.2 | CD14 molecule | F: CACCAAGACGACCCGATGAT | 16 |
| R: AGGGCGATCTGAGCCAATTC | 1 | |||
| MYD88 | XM_042235740.1 | Myeloid differentiation factor 88 | F: TAGGGCAAAAGCCTGAGTATT | 11 |
| R: ACAACTTCAGCCGATAGTTTG | 4 | |||
| TIRAP | XM_042238388.1 | TIR domain containing adaptor protein | F: GCTCAGAGGTGTCTCCCATCCC | 10 |
| R: TGGCACACGCACACATCATAGG | 9 | |||
| TICAM2 | XM_004010183.5 | TIR domain-containing adapter molecule 2 | F: GACGTCAGATTCCAAGCAAT | 12 |
| R: CTTCTTCTGTGTCCTCTTCA | 8 | |||
| IRF3 | XM_004015378.5 | Interferon regulatory factor 3 | F: GAAGGAAGTGTTGCGTTTAGC | 12 |
| R: TGTCTGCCATTGTCTTGAGC | 9 | |||
| IRF5 | XM_042248802.1 | Interferon regulatory factor 5 | F: AATCCTAGTTCTAGTCTCCC | 13 |
| R: TTCATTGGAGGTGAGTCGTG | 1 | |||
| AP-1 | XM_034503172.1 | Transcription factor AP-1 | F: TGAAGGAAGAGCCGCAGAC | 24 |
| R: CCACCTGTTCCCTGAGCATA | 3 | |||
| RIPK1 | XM_027958685.2 | Receptor interacting serine/threonine kinase 1 | F: AACAGAAGGTGCAGTACCAT | 15 |
| R: AGGTCAGCTATCTCTGAACA | 3 | |||
| RELA | XM_027959295.2 | RELA proto-oncogene | F: TCTCATCCCATCTTTGACAACC | 13 |
| R: TGTCCTCTTTCTGCACCTTGT | 3 | |||
| RELB | XM_015100238.3 | RELB proto-oncogene | F: TGGCCTTCCAATCAGGATA | 14 |
| R:AAATGAGCTCAGGAGAAACC | 1 | |||
| IL-1α | NM_001009808.1 | Interleukin 1 alpha | F: GCCAATGATACCGAAGAAGA | 13 |
| R: ATACTTTGATTGAGGGCGTC | 1 | |||
| IL-1β | NM_001009465.2 | Interleukin 1 beta | F: CATCCTTTCATTCATCTTCG | 12 |
| R: GATTTTTGCTCTCTGTCCTG | 0 | |||
| IL-6 | NM_001009392.1 | Interleukin 6 | F: TCAGTCCACTCGCTGTCTCC | 10 |
| R: TCTGCTTGGGGTGGTGTCAT | 7 | |||
| IL-10 | NM_001009327.1 | Interleukin 10 | F: GGTGATGCCACAGGCTGAGAAC | 14 |
| R: GCTCCACCGCCTTGCTCTTG | 3 | |||
| IL-11 | XM_027978781.2 | Interleukin 11 | F: ACGGAGACCACAGCCTGGATTC | 13 |
| R: CAGCCACTGCACATGCCTCAG | 4 | |||
| IL-13 | NM_001082594.1 | Interleukin 13 | F: CCTGATCAGCATCTCCAACTG | 11 |
| R: TTGGTGTCTCGGACGTACT | 7 | |||
| IL-17 | XM_004018887.5 | Interleukin 17 | F: GCGTCTATGAGAACTGCCTCTATGT | 11 |
| R: AGTTCTTGTCCTCAGTAGGTGGGCA | 8 | |||
| TNF-α | NM_001024860.1 | Tumor necrosis factor alpha | F: ATGTGGAGCTGGCGGAGGAG | 12 |
| R: GCAGGCAGAAGAGCGTGGTG | 6 | |||
| IFN-β | XM_027963972.2 | Interferon beta | F: GGCAGTTACCTTCAACT | 12 |
| R: CTGGAGCATCTCATACATG | 8 | |||
| iNOS | XM_042255454.1 | Inducible nitric oxide synthase | F: ATCAAATCCCAAAAGGTGGAC | 17 |
| R: TCTGGAGATGTCTTTACCGT | 6 | |||
| egr-miR-71 | / | GCGAGCACAGAATTAATACGACTCACTATAGG(T)12VN2 | ||
| F: TGAAAGACGATGGTAGTGAGA | ||||
| R: GCGAGCACAGAATTAATACGAC |
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.