Table 1
Lymphocyte proliferation and cytokine production by splenocytes stimulated with rTgUPase.
| Groups # | Lymphocyte SI | Cytokine production (pg/mL)## | |||
|---|---|---|---|---|---|
| IFN-γ | IL-2 | IL-4 | IL-10 | ||
| PBS | 0.65 ± 0.67 | 80.32 ± 78.71 | 87.33 ± 32.88 | 123.42 ± 76.5858 | 74.04 ± 6.34 |
| 10 μg rTgUPase | 1.45 ± 1.49a | 130.37 ± 72.78a | 140.33 ± 65.85a | 358.57 ± 100.199b | 79.33 ± 4.66 |
| 20 μg rTgUPase | 2.44 ± 1.31b | 206.23 ± 67.20b | 152.00 ± 28.32b | 402.70 ± 92.1127b | 83.67 ± 6.71 |
| 30 μg rTgUPase | 2.59 ± 1.36b | 378.14 ± 130.12b | 221.43 ± 46.91b | 481.80 ± 64.949 b | 86.65 ± 5.52 |
| 40 μg rTgUPase | 2.35 ± 0.89b | 354.21 ± 92.57b | 197.29 ± 24.68b | 396.24 ± 59.0392b | 83.64 ± 7.82 |
Splenocytes from mice were harvested 2 weeks after the last immunisation. The results are presented as the arithmetic means ± standard errors of three replicate experiments. Values for IFN-γ are for 96 h, values for IL-2 and IL-4 are for 24 h, and values for IL-10 are for 72 h. a: p < 0.05 vs. control; b: p < 0.01 vs. control.
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