Open Access
Table 1
Primers used for PCR amplification.
| Primers | Primer sequences（5′–3′） | Usage of primers |
|---|---|---|
| MpOBP2exf | ATGAAGTCAATTATTATCTTGGGAGTTTTGCT | Amplify the full-length cDNA gene |
| MpOBP2exr | TTATGTGTACTTGTTGTTGGTCTTGAAG | |
| MpOBP3exf | ATGCGTGGCGTGGTTTTAGCA | |
| MpOBP3exr | TTATTCGTCGTCATGGTGATGGTG | |
| MpOBP8exf | ATGGATTCAAATATAAAATATAT | |
| MpOBP8exr | CTACTGGGTCATTTTCAGAGGC | |
| MpOBP10exf | ATGGCTAAATTTTTGTTGAGC | |
| MpOBP10exr | TTATAGGATTAAATAGGTCTC | |
| MpPBPexf | ATGGTGAGGGTGATACTGAATTACATT | |
| MpPBPexr | TTAAATCATTAACCACATGTCAGGAGAT | |
| MpOBP2-RTexf | CGGAGGCTAAATCGGTACAGAAACG | Real-time PCR |
| MpOBP2-RTexr | CTTGCATCATACGGCCCATGTTTTC | |
| MpOBP3-RTexf | GAAGCAGGAGTCACTAAAG | |
| MpOBP3-RTexr | GTAAATCAGCAGGGAGC | |
| MpOBP8-RTexf | AGTCAAACCAAATATGACCAGCG | |
| MpOBP8-RTexr | TACTGGGTCATTTTCAGAGGCTC | |
| MpOBP10-RTexf | TGCTTATGCTCATTCTGGT | |
| MpOBP10-RTexr | GATGGTGGAG ATAATGCTAA | |
| MpPBP-RTexf | ACATTTTCCTCGGCTTGCTTTTGC | |
| MpPBP-RTexr | TACCCTTCTCTCCTTGAGCCATGTC | |
| Mp18S-RTexf | CGGAGAGGGAGCCTGAGAA | |
| Mp18S-RTexr | CCGGGAGTGGGTAATTTGC | |
| Mpβ-actin-RTexf | TACGCTCTACCTCACGCTATCCTTC | |
| Mpβ-actin-RTexr | CGGCAGTGGTGGTGAAAGAGTATC |
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