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Table 2
List of proteins identified on the 2-DE profiles of Neospora caninum (Nc-1) tachyzoites probed with known bovine sera analysed by mass spectrometry (LC-MS/MS).
| Spot | Accession No. | Protein name | Scorea | M.W.b | No. of peptidec | % coveraged | pI e |
|---|---|---|---|---|---|---|---|
| 1 | CBZ52122.1 | HSP90-like protein, related [N. caninum Liverpool] | 89 | 96,996 | 3 | 6.5 | 4.91 |
| 2 | CBZ53664.1 | Hypothetical protein NCLIV_034460 [N. caninum Liverpool] | 281 | 64,657 | 8 | 18.9 | 4.82 |
| 3 | CEL75932.1 | Ubiquitin carboxyl-terminal hydrolase, putative [T. gondii VEG] | 42 | 247,984 | 2 | 1.1 | 5.75 |
| 4 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 53 | 126,373 | 1 | 0.8 | 9 |
| 5 | CBZ52236.1 | Putative elongation factor 2 [N. caninum Liverpool] | 131 | 92,975 | 5 | 10.1 | 5.93 |
| 6 | CBZ56259.1 | Hypothetical protein NCLIV_066840 [N. caninum Liverpool] | 421 | 79,021 | 10 | 21.2 | 7.48 |
| 7 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 54 | 126,373 | 2 | 2.6 | 9 |
| 8 | CBZ49807.1 | Putative microneme protein MIC4 [N. caninum Liverpool] | 205 | 64,427 | 7 | 17.8 | 6.29 |
| 9 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 57 | 126,373 | 2 | 2.6 | 9 |
| 10 | CEL67492.1 | Inosine-5′-monophosphate dehydrogenase [N. caninum Liverpool] | 395 | 61,250 | 13 | 31 | 7.25 |
| 11 | CBZ53094.1 | Glucose-6-phosphate isomerase, related [N. caninum Liverpool] | 122 | 53,628 | 3 | 8.5 | 6.73 |
| 12 | CBZ50563.1 | Putative dihydrolipoamide branched chain transacylase, E2 subunit [N. caninum Liverpool] | 423 | 69,400 | 10 | 18.4 | 6.2 |
| 13 | CBZ55235.1 | Putative phosphatidylinositol-4-phosphate 5-kinase [N. caninum Liverpool] | 44 | 50,948 | 1 | 5.2 | 6.93 |
| 14 | CBZ54369.1 | Conserved hypothetical protein [N. caninum Liverpool] | 49 | 924,393 | 5 | 0.9 | 6.44 |
| 15 | CBZ52433.1 | Hypothetical protein NCLIV_022220 [N. caninum Liverpool] | 211 | 63,160 | 7 | 15.5 | 8.56 |
| 16 | CEL65811.1 | Putative 2-hydroxyacid dehydrogenase SACOL2296 [N. caninum Liverpool] | 181 | 65,408 | 5 | 13.7 | 6.46 |
| 17 | CBZ51198.1 | gg11844, related [N. caninum Liverpool] | 270 | 49,423 | 7 | 20 | 7.38 |
| 18 | CBZ49859.1 | Actin, related [N. caninum Liverpool] | 347 | 41,881 | 10 | 28.5 | 5.05 |
| 19 | CBZ49859.1 | Actin, related [N. caninum Liverpool] | 111 | 41,881 | 4 | 15.4 | 5.05 |
| 20 | CEL69162.1 | Ubiquitin, putative [N. caninum Liverpool] | 154 | 40,879 | 4 | 18.3 | 4.79 |
| 21 | AAC15250.1 | Surface protein Nc-p43 [N. caninum] | 81 | 42,025 | 2 | 7.8 | 5.49 |
| 22 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 53 | 126,373 | 1 | 0.8 | 9 |
| 23 | CBZ50660.1 | Hypothetical protein NCLIV_011270 [N. caninum Liverpool] | 530 | 44,623 | 15 | 41.6 | 5.61 |
| 24 | CBZ54372.1 | Hypothetical protein NCLIV_048020 [N. caninum Liverpool] | 288 | 36,035 | 7 | 27.1 | 5.63 |
| 25 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 52 | 126,373 | 1 | 0.8 | 9 |
| 26 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 53 | 126,373 | 1 | 0.8 | 9 |
| 27 | CBZ56165.1 | Conserved hypothetical protein [N. caninum Liverpool] | 468 | 33,610 | 9 | 46.9 | 5.6 |
| 28 | CBZ55758.1 | 60S acidic ribosomal protein P0 [N. caninum Liverpool] | 507 | 33,843 | 11 | 51.4 | 5.44 |
| 29 | CBZ54609.1 | Unnamed protein product [N. caninum Liverpool] | 223 | 38,998 | 10 | 33.1 | 7.01 |
| 30 | CBZ49956.1 | Hypothetical protein NCLIV_004400 [N. caninum Liverpool] | 239 | 50,651 | 6 | 18.7 | 8.51 |
| 31 | CBZ54957.1 | Unnamed protein product [N. caninum Liverpool] | 115 | 38,797 | 2 | 8.2 | 5.82 |
| 32 | CBZ54957.1 | Unnamed protein product [N. caninum Liverpool] | 156 | 38,797 | 3 | 11.5 | 5.82 |
| 33 | CBZ49679.1 | Elongation factor 1-alpha, related [N. caninum Liverpool] | 66 | 49,018 | 2 | 4 | 9.02 |
| 34 | CBZ50039.1 | Hypothetical protein NCLIV_005150 [N. caninum Liverpool] | 177 | 50,033 | 4 | 12.7 | 4.7 |
| 35 | CBZ52818.1 | Armadillo/beta-catenin-like repeat-containing protein [N. caninum Liverpool] | 60 | 30,504 | 3 | 15 | 5.01 |
| 36 | CBZ54937.1 | Putative peroxidoxin 2 [N. caninum Liverpool] | 58 | 24,518 | 2 | 8.9 | 5.84 |
| 37 | CBZ52736.1 | Putative Gbp1p protein [N. caninum Liverpool] | 80 | 31,836 | 3 | 15.3 | 9.15 |
| 38 | CBZ54173.1 | Hypothetical protein NCLIV_046050 [N. caninum Liverpool] | 45 | 131,192 | 2 | 2.8 | 6.05 |
| 39 | CBZ52239.1 | Hypothetical protein NCLIV_020250 [N. caninum Liverpool] | 149 | 60,778 | 4 | 7.9 | 5.83 |
| 40 | CBZ51726.1 | Pyruvate kinase, related [N. caninum Liverpool] | 474 | 57,581 | 10 | 28.8 | 6.01 |
| 41 | CEL76893.1 | Dynein 1-beta heavy chain, flagellar inner arm I1 complex, putative [T. gondii VEG] | 79 | 518,185 | 7 | 2.5 | 6.05 |
| 42 | CEL76893.1 | Dynein 1-beta heavy chain, flagellar inner arm I1 complex, putative [T. gondii VEG] | 37 | 518,185 | 2 | 0.5 | 6.05 |
| 43 | CBZ54957.1 | Unnamed protein product [N. caninum Liverpool] | 203 | 38,797 | 5 | 14.6 | 5.82 |
| 44 | CEL66738.1 | Elongation factor Tu, putative [N. caninum Liverpool] | 156 | 59,096 | 7 | 17.6 | 8.23 |
| 45 | CEL71761.1 | Hypothetical protein BN1205_038970 [T. gondii VEG] | 57 | 50,170 | 1 | 3.3 | 5.15 |
| 46 | CBZ50660.1 | Hypothetical protein NCLIV_011270 [N. caninum Liverpool] | 202 | 44,623 | 5 | 17.2 | 5.61 |
| 47 | CBZ53299.1 | Conserved hypothetical protein [N. caninum Liverpool] | 63 | 61,681 | 3 | 9.9 | 4.78 |
| 48 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 45 | 126,373 | 1 | 0.8 | 9 |
| 49 | CBZ53399.1 | Putative serine-threonine phosphatase 2C [N. caninum Liverpool] | 261 | 35,552 | 7 | 29 | 5.35 |
| 50 | CBZ53399.1 | Putative serine-threonine phosphatase 2C [N. caninum Liverpool] | 120 | 35,552 | 4 | 18.1 | 5.35 |
| 51 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 53 | 126,373 | 1 | 0.8 | 9 |
| 52 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 53 | 126,373 | 1 | 0.8 | 9 |
| 53 | CEL76857.1 | corA-like Mg2 transporter domain-containing protein [T. gondii VEG] | 46 | 126,373 | 2 | 2.6 | 9 |
| 54 | CAA06661.1 | p36 protein [N. caninum] | 457 | 33,049 | 11 | 37.3 | 7.89 |
| 55 | CAA06661.1 | p36 protein [N. caninum] | 136 | 33,049 | 4 | 19.1 | 7.89 |
| 56 | CBZ51110.1 | Putative Hsp20/alpha crystalline domain-containing protein [N. caninum Liverpool] | 210 | 29,682 | 7 | 29.5 | 5.56 |
| 57 | CBZ53437.1 | Catalase (EC 1.11.1.6), related [N. caninum Liverpool] | 129 | 58,700 | 5 | 13.6 | 6.75 |
| 58 | CBZ54609.1 | Unnamed protein product [N. caninum Liverpool] | 1235 | 38,998 | 24 | 59.1 | 7.01 |
| 59 | CBZ51119.1 | Glyceraldehyde 3-phosphate dehydrogenase, related [N. caninum Liverpool] | 425 | 36,452 | 9 | 39.7 | 6.83 |
| 60 | CBZ51119.1 | glyceraldehyde 3-phosphate dehydrogenase, related [N. caninum Liverpool] | 518 | 36,452 | 11 | 40.3 | 6.83 |
| 61 | CEL68024.1 | Hypothetical protein BN1204_038021 [N. caninum Liverpool] | 49 | 191,022 | 4 | 2.4 | 5.03 |
| 62 | CBZ49679.1 | Elongation factor 1-alpha, related [N. caninum Liverpool] | 59 | 49,018 | 1 | 2.5 | 9.02 |
| 63 | CBZ49679.1 | Elongation factor 1-alpha, related [N. caninum Liverpool] | 866 | 49,018 | 21 | 52 | 9.02 |
| 64 | CBZ49679.1 | Elongation factor 1-alpha, related [N. caninum Liverpool] | 655 | 49,018 | 17 | 43.8 | 9.02 |
| 65 | CEL78445.1 | ATP-dependent DNA helicase II, 70 kDa subunit, putative [T. gondii VEG] | 39 | 94,541 | 2 | 3.1 | 4.95 |
| 66 | CEL78039.1 | Pantothenate kinase, putative [T. gondii VEG] | 59 | 125,264 | 4 | 6.9 | 9.06 |
| 67 | CEL71084.1 | Protein phosphatase 2C, putative [N. caninum Liverpool] | 123 | 46,413 | 3 | 9.2 | 5.94 |
| 68 | CBZ52637.1 | Hypothetical protein NCLIV_024250 [N. caninum Liverpool] | 162 | 16,436 | 4 | 17.7 | 10.4 |
| 69 | CBZ53255.1 | Rcn2-prov protein, related [N. caninum Liverpool] | 227 | 39,265 | 6 | 20 | 4.65 |
| 70 | CBZ53608.1 | Heat shock protein 70, related [N. caninum Liverpool] | 189 | 72,613 | 4 | 5.4 | 5.07 |
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