Figure 6
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rSmPGM binds to plasminogen and promotes plasmin generation. (A) SmPGM activity of heat-inactivated SmPGM (HI-SmPGM, red line) compared to non-heat-treated SmPGM (blue line) ***p < 0.001 (two-way ANOVA with Bonferroni post-test). (B) Plasminogen (PLMG, at increasing concentration) binding to purified rSmPGM (0.5 μg, purple line) versus heat-inactivated rSmPGM (HI-SmPGM, 5.0 μg, red line) or control protein (BSA, 0.5 μg, green line) detected by ELISA. Significant differences between PLMG binding to rSmPGM versus heat-inactivated (HI) rSmPGM or BSA at all concentrations of PLMG are denoted by ***p < 0.001 (two-way ANOVA with Bonferroni post-test). (C) Plasmin activity detected in the presence of tPA and PLMG alone (green line) or supplemented with increasing amounts of purified SmPGM (0.5–5.0 μg, as indicated) or BSA (1.0 μg, blue line) or HI-SmPGM (5.0 μg, orange line) over time. Significant differences at all time points beyond 20 min compared to controls are denoted by ***p < 0.001 (two-way ANOVA with Bonferroni post-test).
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