De novo transcriptome assembly and identification of G-Protein-Coupled-Receptors (GPCRs) in two species of monogenean parasites of fish

Open Access

Table 1

Assembly and ORF statistics generated for Rhabdosynochus viridisi and Scutogyrus longicornis.

	*S. longicornis*							*R. viridisi*
	Unfiltered	First filtering		Second filtering		Third filtering		Unfiltered	First filtering		Second filtering		Third filtering
	Assembly	Assembly	ORF	Assembly	ORF	Assembly	ORF	Assembly	Assembly	ORF	Assembly	ORF	Assembly	ORF
# sequences ≥0 bp	51,817	49,873	22,197	48,775	21,241	47,814	19,291	294,928	269,985	55,141	267,005	47,497	264,494	41,059
# sequences ≥1000 bp	20,258	19,127	6663	14,119	6464	13,169	5997	63,638	54,716	8818	52,818	7067	51,163	5670
# sequences ≥3000 bp	10,291	9594	455	5361	445	4852	408	33,686	28,665	678	23,965	560	20,032	458
# sequences	82,366	78,594	29,315	68,255	28,150	65,835	25,696	392,252	353,366	64,637	343,788	55,124	335,689	47,187
# genes	48,086	46,372	13,179	45,995	12,821	45,194	12,020	313,109	284,085	30,291	280,648	26,854	277,651	23,857
Total length (bp)	111,966,103	105,481,027	23,584,962	70,992,660	22,763,481	66,069,113	20,893,392	484,764,415	422,751,986	40,917,888	369,417,136	34,017,756	329,849,780	28,346,136
GC (%)	42.56	42.49	46.38	42.49	46.44	42.46	4649	45.72	45.75	51.2	45.34	51.27	44.92	51.35
Average length of sequences	1359.37	1342.1	804.54	1040.11	808	1003.56	813	1235.85	1196.36	633.04	1074	617	982	600
N50	2906	2874	1020	2197	1029	2114	1038	3567	3496	750	2636	711	2132	666

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