Phylogenetic analysis of Rhabdosynochus viridisi and Scutogyrus longicornis orphan GPCRs. The midpoint-rooted phylogenetic tree was constructed using 1000 replicates of the approximate likelihood ratio test (similar to the Shimodaira-Hasegawa test). The LG+F+R3 model was implemented. The colored boxes indicate the log10-transformed e-values obtained from the alignment of the R. viridisi and S. longicornis sequences against sequences of different taxa using the NCBI database (Tre, Trematoda; Ces, Cestoda; Rha, Rhabditophora; Lop, Lophotrochozoa; Spi, Spiralia; Pro, Protostomia; Bil, Bilateria; Ver, Vertebrata); the species to which the sequence belongs (R. viridisi or S. longicornis); and the type of receptor inferred from phylogenetic similarity to reference sequences.