Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

Open Access

Table 3

Evaluation of fecal load of Blastocystis in human samples based on the established quantification curve (set in the range of 10⁰ to 10⁵ cells per 1 qPCR reaction).

Estimated fecal protist load¹	Number of samples/Number of positive samples	Ct value range
10¹–10²	18/83	28–32
10³–10⁴	13/83	21–27
10⁵ –10⁶	52/83	15–20

Number of cells per 1 qPCR reaction.

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