Open Access
Table 1
Primers used for RT-qPCR in this study.
| Target | Source (Genbank) | Target gene | Primer sequence (5′ – 3′) | Amplicon length (bp) | Average Tm (°C) | Amplification efficiency (%) |
|---|---|---|---|---|---|---|
| TrxR-L_fw | XM_004353581 | thioredoxin reductase 1 | GGATTCGACCAACAGCTCG | 212 | 59.5 | 96.3 |
| TrxR-L_rev | cytoplasmic, putative | GGATTCGACCAACAGCTCG | 59.5 | |||
| TrxR-S_fw | M_004351629 | thioredoxin-disulfide | CTCTCGAACCCCAAGATC | 182 | 56.3 | 97.3 |
| TrxR-S_rev | reductase | CACCTGACCATTCAGGAAC | 57.5 | |||
| Trx-1_fw | XM_004335461 | thioredoxin-1, putative | GGACTTCTTTGCCACGTG | 184 | 56.3 | 95.5 |
| Trx-1_rev | GCACCTTGCTGCCGTTC | 57.3 | ||||
| Prx-2_fw | XM_004333592 | peroxiredoxin 2 | GACACACCTACCGTGGTC | 226 | 58.4 | 77.9 |
| Prx-2_rev | GTTGACCTTCTCGAAGTAGG | 58.4 | ||||
| Prx-3_fw | XM_004348492 | 2cys peroxiredoxin | CAACGACTTGCCAGTGGG | 157 | 58.4 | 94.1 |
| Prx-3_rev | CCTTGAAGTACTCCTTGGAC | 58.4 | ||||
| Grx-1_fw | XM_004339700 | glutaredoxin, putative | CGCCAAGAACACCGTTATG | 203 | 57.5 | 90.3 |
| Grx-1_rev | CCAATGTGCTCACTGTCGATG | 56.3 | ||||
| Grx-2_fw | XM_004339719 | glutaredoxin, putative | GGAGATGAGAGCGTTCAG | 184 | 56.3 | 98.8 |
| Grx-2_rev | CTCTTGGCCTGCATCTCG | 58.4 | ||||
| GR_fw | XM_004338198 | glutathione-disulfide | CGACACTCTCTACAACAACC | 149 | 59.5 | 90.6 |
| GR_rev | reductase | CTTCTCGTCACGCTTGGAC | 61.6 | |||
| Gpx_fw | XM_004335951 | glutathione peroxidase Hyr1 | CTGCAACCAGTTCGGCAG | 193 | 58.4 | 91.7 |
| Gpx_rev | CGAAGTTCCACTTGATGCG | 57.5 | ||||
| 18S_fw | 18S-rRNA-gene | CCCAGATCGTTTACCGTGAA | 180 | 58.4 | 97.5 | |
| 18S_rev | TAAATATTAATGCCCCCAACTATCC | 60.9 | ||||
| HPRT_fw | XM_004337011 | hypoxanthine-guanine phosphoribosyltransferase | GGAGCGGATCGTTCTCTG | 201 | 58.4 | 102 |
| HPRT_rev | ATCTTGGCGTCGACGTGC | 58.4 |
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