Impact of RNAi knockdown of TbKINX1B or TbBILBO1 in PCF on expression and localization. A. Minor phenotypes were observed in TbKINX1B^RNAi cells induced for 24 h to 72 h. (a) The flagella were labelled with anti-PFR antibody (L8C4). (b) The FPC structures were labelled with anti-TbBILBO1 antibody. (c) The axonemes were labelled with anti-TbSAXO antibody (mAb25). (d) The FAZ structure was labelled with the anti-FAZ antibody (L3B2). Scale bars 5 μm. B. Expression level of TbKINX1B upon RNAi down-regulation of TbBILBO1. Immunoblotting of TbKINX1B, TbBILBO1 and Tubulin in whole cell protein extracts (WC), detergent-extracted cytoskeleton fraction (CSK), and soluble fraction (S) of lysed TbBILBO1^RNAi non-induced (NInd) or induced for 36H (Ind) cells.