TbKINX1B can hydrolyse ATP in vitro. A. ATPase activity using the ELIPA in vitro assay. Kinesin activity was measured by the amount of generated phosphate per minute, in the presence of taxol-stabilized microtubules and ATP. The human kinesin heavy chain motor domain (KHC^MD) is the positive control kinesin in presence of MTs (+MTs, black line) or negative control in the absence of MT (−MTs, grey line), the TbKINX1B^MD (green), TbKINX1B^MD deleted for the ATPase domain (ΔP-loop^MD, red line), and _6HisTRX purified protein (yellow line), (n = 3). B. Recombinant TbKINX1B proteins purification. Western blot analysis (upper panel) and Coomassie-stained SDS-PAGE (lower panel) of the purification of the _Trx-6HisTbKINX1B^MD (A), _Trx-6HisTbKINX1B-ΔP-loop^MD (B), and Trx-6His (C) proteins. The recombinant proteins were immunolabelled using anti-Histidine tag. Abbreviations: Total (T), non-induced (NI), induced (IND), supernatant (S), flow-through (FT), wash (W, different wash fractions numbered), Elution (E, fractions numbered).