Microtubule and FPC localization of TbKINX1B depends on its motor domain and BILBO1-binding domain respectively. A. Immuno-precipitation of TbKINX1B and immuno-detection by western blotting of TbKINX1B and TbBILBO1 on input (I), flow-through (FT), wash (W), and elution (E) fractions. B. Induction of the expression of TbKINX1B_myc, TbKINX1B_myc^MD or TbKINX1B_myc^B1BD does not affect PCF cell growth, as compared to WT (n = 3). C. Western blot of PCF WT cells and cells non-induced (–) or tetracycline-induced 24 h, 48 h, 72 h for the expression of TbKINX1B_myc (151 KDa), TbKINX1B_myc^MD (62 KDa) or TbKINX1B_myc^B1BD (28 KDa). Expressed proteins were detected using anti-myc antibody and using PFR2 (L8C4) as a loading control. No alteration on TbBILBO1 protein levels was observed. D. Co-immunolabelling on detergent-extracted cells of TbKINX1B_myc and TbKINX1B_myc^B1BD proteins (green) with TbBILBO1 (red), and of TbKINX1B_myc^MD (green) with Tubulin (red).