Table 3
Sensitivity and specificity estimated after a comparative study of commercial PCR multiplex for each targeted gastrointestinal protist.
| Technique | No of samples tested | No of samples and corresponding sensitivity and specificity |
Overall Se (CI) | Overall Sp (CI) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C. parvum/hominis
|
G. intestinalis
|
E. histolytica
|
D. fragilis
|
Blastocystis sp.. |
|||||||||
| Se (CI)* | Sp (CI)** | Se (CI) | Sp (CI) | Se (CI) | Sp (CI) | Se (CI) | Sp (CI) | Se (CI) | Sp (CI) | ||||
| G-DiaParaTrio® (Diagenode) | 184 | 100% (26/26) | 100% (156/156) | 92.5% (82.5–100) (37/40) | 100% (144/144) | 71.4% (42.9–100) (5/7) | 100% (177/177) | NA | NA | NA | NA | 93.2% (87.4–99) (68/73) | 100% (477/477) |
| Allplex® (Seegene) | 184 | 100% (26/26) | 100% (156/156) | 97.5% (92.5–100) (39/40) | 100% (144/144) | 85.7% (57.1–100) (6/7) | 100% (177/177) | 100% (4/4) | 94.4% (90.6–97.2) (170/180) | 95.3% (89.1–100) (61/64) | 97.5% (94.2–100) (117/120) | 96.5% (93.4–99.5) (136/141) | 98.3% (97.4–99.2) (764/777) |
| Rida®gene (r-biopharm) | 184 | 92.3% (80.8–100) (24/26) | 100% (156/156) | 92.5% (82.5–100) (37/40) | 99.3% (97.9–100) (143/144) | 100% (7/7) | 100% (177/177) | 25% (0–75) (1/4) | 98.3% (96.1–100) (170/180) | NA | NA | 89.6% (82.8–96.4) (69/77) | 98.3% (95.5–100) (646/657) |
| Composite reference method*** | 184 | 100% (26/26) | 100% (156/156) | 92.5% (82.5–100) (37/40) | 100% (144/144) | 42.9% (14.3–85.7) (3/7) | 92.9% (79.4–100) (13/14) | 25% (0–75) (1/4) | 100% (180/180) | 26.6% (15.6–37.5) (17/64) | 100% (120/120) | 59.6% (51.5–67.7) (84/141) | 99.8% (99.5–100) (613/614) |
| Final parasitological diagnosis§ | 26 | 40 | 7 | 4 | 64 | 141 | |||||||
Se: sensitivity, Sp: Specificity, CI: 95% confidence interval, NA: not applicable.
Sensitivity corresponds to the percentage of true positive results obtained for the targeted pathogen by the tested technique and was calculated as follows: Se = (number of positive results observed/overall positive results). The 95% confidence interval was estimated with a binomial law.
Specificity corresponds to the percentage of true negative results obtained by the tested technique and was calculated as follows: Sp = (number of negative results observed/overall negative results) */100. The 95% confidence interval was estimated with a binomial law.
The composite reference method was composed of a microscopic examination based on iodine-stained mount, Bailanger’s diphasic concentration method and modified Ziehl–Neelsen staining associated with E. histolytica-specific adhesion detection.
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