Open Access

Table 2

Characteristics of the three commercial multiplex PCR kits included in this retrospective comparative study for the assessment of gastrointestinal protists diagnosis.

Commercial kit Manufacturer Amplification technology Thermocycler Targets Targeted genes UDG system Internal inhibition control# Dedicated analysis software
Allplex® GI parasite Seegene MuDT Bio-Rad CFX96* Blastocystis sp. NA Yes Extraction and amplification control Yes
Cryptosporidium spp.
Cyclospora cayetanensis
Dientamoeba fragilis
Entamoeba histolytica
Giardia intestinalis
G-DiaParaTrio Diagenode Taqman Bio-Rad CFX96 C. parvum/hominis Segment A No Extraction and amplification control No
Applied Biosystems ABI 7500* Entamoeba histolytica 18S rRNA
Roche LC 480 Giardia intestinalis 18S rRNA
Qiagen Rotor-gene
RIDAGENE parasitic stool panel R-Biopharm Taqman Bio-Rad CFX96 Cryptosporidium spp. ITS1-18S No Extraction and amplification control No
Melting curve Applied Biosystems ABI 7500* Dientamoeba fragilis
Roche LC 480 Entamoeba histolytica
Qiagen Rotorgene Giardia intestinalis
Cepheid Smartcycler
Abbott m2000rt
Stratagene Mx3000P, Mx3005P

MuDT Technologies is a real-time PCR technology that enables the detection of multiple targets with a Ct value in a single channel. NA: not available. UDG system: Uracil-DNA glycosylase system.


Recommended thermocycler used in this study.


Internal inhibition control was added into the reaction mixture before DNA amplification as recommended by the manufacturers to be used only as an amplification control in this study based on DNA extracts from a retrospective multicentric stool samples collection.

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