Table 2
Characteristics of the three commercial multiplex PCR kits included in this retrospective comparative study for the assessment of gastrointestinal protists diagnosis.
| Commercial kit | Manufacturer | Amplification technology | Thermocycler | Targets | Targeted genes | UDG system | Internal inhibition control# | Dedicated analysis software |
|---|---|---|---|---|---|---|---|---|
| Allplex® GI parasite | Seegene | MuDT | Bio-Rad CFX96* | Blastocystis sp. | NA | Yes | Extraction and amplification control | Yes |
| Cryptosporidium spp. | ||||||||
| Cyclospora cayetanensis | ||||||||
| Dientamoeba fragilis | ||||||||
| Entamoeba histolytica | ||||||||
| Giardia intestinalis | ||||||||
| G-DiaParaTrio | Diagenode | Taqman | Bio-Rad CFX96 | C. parvum/hominis | Segment A | No | Extraction and amplification control | No |
| Applied Biosystems ABI 7500* | Entamoeba histolytica | 18S rRNA | ||||||
| Roche LC 480 | Giardia intestinalis | 18S rRNA | ||||||
| Qiagen Rotor-gene | ||||||||
| RIDAGENE parasitic stool panel | R-Biopharm | Taqman | Bio-Rad CFX96 | Cryptosporidium spp. | ITS1-18S | No | Extraction and amplification control | No |
| Melting curve | Applied Biosystems ABI 7500* | Dientamoeba fragilis | ||||||
| Roche LC 480 | Entamoeba histolytica | |||||||
| Qiagen Rotorgene | Giardia intestinalis | |||||||
| Cepheid Smartcycler | ||||||||
| Abbott m2000rt | ||||||||
| Stratagene Mx3000P, Mx3005P |
MuDT Technologies is a real-time PCR technology that enables the detection of multiple targets with a Ct value in a single channel. NA: not available. UDG system: Uracil-DNA glycosylase system.
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